QUANTITATIVE GENETIC ANALYSIS OF SIGNAL TRANSDUCTION

信号转导的定量遗传分析

• 批准号: 6387194
• 负责人: GREGORY C GIBSON
• 金额: $ 18.31万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6387194
• 关键词: Drosophilidae; biological signal transduction; gene expression; genetic polymorphism; genotype; growth /development; linkage disequilibriums; linkage mapping; molecular biology information system; molecular genetics; morphology; phenotype; polymerase chain reaction; protein tyrosine kinase; quantitative trait loci; statistics /biometry; visual photoreceptor

The long-term objective of the proposed study is to establish how molecular variation in specific proteins involved in a signal transduction pathway contributes to phenotypic variation. The project focuses on the developmental pathways associated with wing shape and photoreceptor variation in Drosophila. The general approach is to use genetic "tester" backgrounds that bear selected hypo- and hypermorphic mutations shown by Dr. Gibson to reveal modifiers of expression. He proposes that these modifiers are genes in the same signal transduction pathway that contribute to potential subtle perhaps cryptic standing genetic variation in natural populations. In these "sensitized" backgrounds measured phenotypic effects are mapped to the candidate genes of the associated EGF-Ras pathway using association or linkage disequilibrium mapping, as has been used to define QTLs for bristle variation in natural populations of D. melanogaster. The objective at this point is not to identify specific mutations responsible for functional variation yet, but to quantify relative effects of different loci in the pathway.

拟议研究的长期目标是确定如何 参与信号转导的特定蛋白质的分子变异 途径有助于表型变异。该项目的重点是 与翅膀形状和感光器变异相关的发育途径 在果蝇中。一般方法是使用基因“测试仪”背景 吉布森博士展示了所选择的低形态和超形态突变，以揭示 表达修饰语。他提出这些修饰因子是同一个基因中的基因。 有助于潜在微妙或神秘的信号转导途径 自然群体中存在的遗传变异。在这些“敏感”的 背景测量的表型效应被映射到候选基因 使用关联或连锁不平衡的相关 EGF-Ras 通路 作图，已用于定义天然鬃毛变异的 QTL 黑腹果蝇种群。此时的目标不是确定 导致功能变异的特定突变尚未确定，但需要量化 途径中不同基因座的相对影响。