FUNCTIONS OF EBNA-1 IN THE STABLE REPLICATION OF EBV

EBNA-1在EBV稳定复制中的作用

• 批准号: 6653091
• 负责人: Ashok A Aiyar
• 金额: $ 14.89万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-30 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653091
• 关键词: Burkitt's lymphoma; DNA replication origin; Epstein Barr virus; Saccharomyces cerevisiae; human tissue; neoplasm /cancer genetics; plasmids; tissue /cell culture; virus antigen; virus genetics; virus protein; virus replication

I will continue ongoing studies designed to characterize the mechanism by which the Epstein-Barr nuclear antigen 1 (EBNA-1) protein of Epstein-Barr virus (EBV) facilitates the stable replication of plasmids that bear oriP, EBV's plasmid origin of replication. Together my post-doctoral mentor, Dr. Bill Sugden, and I have demonstrated that oriP-plasmids are synthesized directly by the cell, and that EBNA-1 functions post- synthetically to stabilize oriP-plasmids. Our work also demonstrates that human cells can actively eliminate extra- chromosomal DNAs. This application experimentally addresses the process by which human cells eliminate plasmids, by defining when it occurs in the cell-cycle, and understanding the mechanism by which those viruses that maintain their genomes as plasmids in human cells overcome it. It also experimentally addresses the mechanism by which oriP-plasmids are stabilized so that they can be partitioned faithfully to daughter cells, and the contributions of EBNA-1 to these processes.

我将继续进行旨在表征Epstein-Barr病毒（EBV）的Epstein-Barr核抗原1（EBNA-1）蛋白（EBV）促进具有EBV Orip（EBV复制的质粒起源的质粒）的稳定复制的机制。 我的博士后导师比尔·苏格登（Bill Sugden）博士一起证明了orip质粒是由细胞直接合成的，并且EBNA-1在合成后起作用以稳定Orip质量。 我们的工作还表明，人类细胞可以主动消除外染色体DNA。 该应用在实验中通过定义在细胞周期中何时发生的人类细胞消除质粒的过程，并理解那些将其基因组作为人类细胞中质粒保持质粒的机制。 它还在实验上解决了稳定Orip质粒的机制，以便可以将它们忠实地分配给子女细胞，以及EBNA-1对这些过程的贡献。

项目成果

The use of CLUSTAL W and CLUSTAL X for multiple sequence alignment.

• DOI: 10.1385/1-59259-192-2:221
• 发表时间: 2000
• 期刊: Methods in molecular biology
• 作者: Ashok Aiyar

Cholesterol Supplementation During Production Increases the Infectivity of Retroviral and Lentiviral Vectors Pseudotyped with the Vesicular Stomatitis Virus Glycoprotein (VSV-G).

• DOI: 10.1016/j.bej.2008.12.004
• 发表时间: 2009-05-15
• 期刊: Biochemical engineering journal
• 影响因子: 3.9
• 作者: Chen Y;Ott CJ;Townsend K;Subbaiah P;Aiyar A;Miller WM
• 通讯作者: Miller WM