SENSING SUPERFUND CHEMICALS WITH RECOMBINANT SYSTEMS

使用重组系统传感超级化学品

• 批准号: 6301509
• 负责人: Sylvia Daunert
• 金额: $ 14.25万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6301509
• 关键词: aromatic hydrocarbon receptor; bacterial genetics; biosensor device; environmental contamination; environmental toxicology; halobiphenyl /halotriphenyl compound; hazardous substances; method development; monitoring device; reporter genes; toxin metabolism

The resistance of PCBs to chemical and biological breakdown processes in nature makes them persistent in the environment. Many of the analytical techniques presently used for monitoring pollutants such as PCBs require expensive equipment and extensive pretreatment of environmental samples. Recognition of the difficulties inherent in classical analytical techniques has spurred the development of novel sensing systems that use a biological component as the sensory element. In that respect, the goal of this project is to develop their breakdown products (e.g., hydroxylated PCBs, chlorocatechols, etc.) that can be found in hazardous waste sites. The biosensing systems proposed are based on the use of recombinant bacteria that incorporate that catabolic pathways for PCBs along with the expression of reporter genes. In addition, sensors based on mammalian and test cells will also be developed by constructing systems where the expression of a reporter protein is coupled to the binding of the species of interest with the aromatic hydrocarbon receptor. Potential advantages of our proposed biosensory technology include economy, portability and the possibility of identifying and quantifying specific compounds directly in complex mixtures found in soil and groundwater samples.

PCB 对自然界化学和生物分解过程的抵抗力使其能够在环境中持久存在。目前用于监测 PCB 等污染物的许多分析技术需要昂贵的设备和对环境样品的大量预处理。对经典分析技术固有困难的认识刺激了使用生物成分作为传感元件的新型传感系统的开发。在这方面，该项目的目标是开发可在危险废物场中找到的分解产品（例如羟基化 PCB、氯儿茶酚等）。所提出的生物传感系统基于重组细菌的使用，该细菌将 PCB 的分解代谢途径与报告基因的表达结合起来。此外，基于哺乳动物和测试细胞的传感器也将通过构建系统来开发，其中报告蛋白的表达与感兴趣的物种与芳香烃受体的结合相耦合。我们提出的生物传感技术的潜在优势包括经济性、便携性以及直接识别和量化土壤和地下水样品中复杂混合物中特定化合物的可能性。