METAL SEQUESTERING BY MICROORGANISMS IN AND FROM AQUATIC ENVIRONMENTS

水生环境中微生物的金属螯合

• 批准号: 6347533
• 负责人: THOMAS E JENSEN
• 金额: $ 6.38万
• 依托单位: HERBERT H. LEHMAN COLLEGE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6347533
• 关键词: Staphylococcus aureus; X ray microscopy; active sites; alginates; alternatives to animals in research; atomic absorption spectrometry; bacteria; carrageenan; cell components; cell morphology; cell wall; cytoplasm; heavy metals; mathematical model; microorganism metabolism; polyphosphates; scanning electron microscopy; transmission electron microscopy; water environment; water pollution

We propose research will test the hypothesis where we propose that microbial polyphosphate bodies (PPBs) are the main sites of metal sequestering. We will use our technology of a transmission electron microscope (TEM) with a scanning transmission mode (STGM) and an associated energy dispersive spectrometer (EDX) to determine which cell component sequesters the most metal. Spectra obtained with the spot mode setting of the STEM from different cell components will be obtained. The cell components to be analyzed are capsule (sheath) cell wall. cytoplasm, and PPBs. We will calculate the volume of the cell each of these components has using the appropriate mathematical formula for the shape. The amount of the metal in each cell area will be determined quantitatively using the Princeton Gamma Tech IMIX-EDX. From this data we will calculate amount of metal sequestered in each cell area or component. We propose using for test organisms (1) Plectonema boryanum, (2) Staphylococcus aureus, (3) our isolates from waste water and (4) an activated sludge biomass. If our hypothesis is sustained we will develop a metal removing system where cells with large numbers of PPBs are enrobed in either kappa-carrageenan or Na-alginate. The aerobic system will be converted to an anaerobic environment. This PPB should be dismantled with the metal being released into the supernant. This should allow the metal to be concentrated many times.

我们建议研究将检验我们提出的假设，即微生物多磷酸盐体（PPB）是金属螯合的主要位点。我们将使用具有扫描透射模式 (STGM) 的透射电子显微镜 (TEM) 技术和相关的能量色散光谱仪 (EDX) 来确定哪种电池组件螯合最多的金属。将获得通过 STEM 点模式设置从不同细胞成分获得的光谱。待分析的细胞成分是荚膜（鞘）细胞壁。细胞质和 PPB。我们将使用适当的形状数学公式来计算每个组件所具有的单元体积。每个单元区域中的金属量将使用 Princeton Gamma Tech IMIX-EDX 进行定量测定。根据这些数据，我们将计算每个单元区域或组件中螯合的金属量。我们建议使用 (1) Plectonema boryanum、(2) 金黄色葡萄球菌、(3) 我们从废水中分离出来的微生物以及 (4) 活性污泥生物质作为测试微生物。如果我们的假设成立，我们将开发一种金属去除系统，其中含有大量 PPB 的细胞被 kappa-卡拉胶或海藻酸钠包裹。有氧系统将转变为无氧环境。应拆除该 PPB，并将金属释放到上清液中。这应该可以使金属多次浓缩。