Apoptosis of ESNLCs

ESNLC的凋亡

• 批准号: 6565293
• 负责人: EUGENE M JOHNSON
• 金额: $ 17.53万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6565293
• 关键词: apoptosis; cell line; developmental neurobiology; embryonic stem cell; laboratory mouse; nervous system regeneration; spinal cord injury; stem cell transplantation; terminal nick end labeling

Description: (from the abstract) Apoptotic cell death occurs in the nervous system during normal and in pathological situations. The PI's preliminary data indicate that apoptosis has a major role in the ES cell graft after transplant into the injured rodent spinal cord. The extent of cell death underlie this process are currently unknown. The goal of this proposal is to apoptotic cell death of ES cell-derived neural lineage cells (ESNLCs). In relevant stages of development. In the second, he will characterize the apoptosis-inducing activity of the syrinx fluid which accumulates in the spinal apoptosis-inducing activity, and determine the changes in this activity as a function of time post-injury. Third, he will utilize the paradigms developed in to the molecular mechanisms of apoptosis and to develop cell clines that are resistant to apoptosis. These experiments should help to define the optimal molecules that are responsible for apoptosis induction seen after the success of ESNLC transplants.

描述：（摘自摘要）在正常和病理情况下，神经系统中都会发生细胞凋亡。 PI 的初步数据表明，ES 细胞移植到受伤的啮齿动物脊髓后，细胞凋亡在其中发挥着重要作用。这一过程中细胞死亡的程度目前尚不清楚。该提案的目标是使 ES 细胞衍生的神经谱系细胞 (ESNLC) 发生凋亡。处于相关发展阶段。在第二部分中，他将表征在脊髓细胞凋亡诱导活性中积累的注射液的细胞凋亡诱导活性，并确定该活性随损伤后时间的变化。第三，他将利用在细胞凋亡分子机制中开发的范例并开发抵抗细胞凋亡的细胞系。这些实验应该有助于确定 ESNLC 移植成功后诱导细胞凋亡的最佳分子。