CORE--IMMUNOPATHOLOGY

核心--免疫病理学

• 批准号: 6565267
• 负责人: PATRICIA A GONNELLA
• 金额: $ 29.59万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6565267
• 关键词: biomedical facility; cytokine; electron microscopy; immunocytochemistry; immunofluorescence technique; light microscopy; pathology

The immunopathology core will provide investigators within the Program Project with the ability to correlate functional studies with in situ morphological analysis of the types of cells and cytokines involved in local inflammation and immune response. 1) The following specialized techniques are available through the core facility. a) tissue processing and embedding b) cryosectioning: light microscopic and electron microscopic c) immunohistochemistry d) immunofluorescence staining and microscopy e) immunofluorescence staining and microscopy f) photography 2) The aforementioned techniques will be used to: a) examine cytokine regulation in the gut and brain in autoimmune disease b) correlate the effect of stimulation with various antigenetic peptides with changes in cytokine expression in gut associated lymphoid tissue and the central nervous system.

免疫病理学核心将为该项目的研究人员提供将功能研究与参与局部炎症和免疫反应的细胞和细胞因子类型的原位形态学分析相关联的能力。 1) 通过核心设施可以使用以下专业技术。 a) 组织处理和包埋 b) 冷冻切片：光学显微镜和电子显微镜 c) 免疫组织化学 d) 免疫荧光染色和显微镜检查 e) 免疫荧光染色和显微镜检查 f) 摄影 2) 上述技术将用于： a) 检查细胞因子在细胞内的调节b) 将各种抗原肽的刺激效果与肠道相关淋巴组织和中枢神经系统中细胞因子表达的变化联系起来。