An Artificial Sequence-based Protein Receptor Array

基于人工序列的蛋白质受体阵列

• 批准号: 6584365
• 负责人: Chin-Shiou Huang
• 金额: $ 110.98万
• 依托单位: ASPIRA BIOSYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6584365
• 关键词: acidity /alkalinity; bioengineering /biomedical engineering; biotechnology; chemical structure function; crosslink; fluorescence spectrometry; fluorescent dye /probe; gene expression; high throughput technology; informatics; intermolecular interaction; intracellular transport; microarray technology; molecular shape; peptide chemical synthesis; polymers; posttranslational modifications; protein purification; protein quantitation /detection; protein sequence; proteomics; receptor; technology /technique development

DESCRIPTION (provided by applicant): Our goal under this Phase II SBIR is to demonstrate that Protein Print TM Arrays can be effectively used for parallel expression profiling of biomarkers and novel therapeutic targets. As part of this work we will generate a commercially viable biomarker array for cancer research and diagnostics and optimize binding and detection conditions. During Phase I, we successfully generated arrays against six proof-of-principle proteins with diverse functions and properties. The cavities have high affinities (Kd ~ l nM) comparable to very good antibodies and specificity at the single amino acid level. This technology represents a breakthrough in our ability to study novel proteins with the potential to resolve the bottleneck in converting the wealth of genomic information into improved drug discovery.

描述（由申请人提供）： 我们在此 II 期 SBIR 下的目标是证明 Protein Print TM Arrays 可有效用于生物标志物和新型治疗靶点的并行表达分析。作为这项工作的一部分，我们将为癌症研究和诊断生成商业上可行的生物标志物阵列，并优化结合和检测条件。在第一阶段，我们成功生成了针对六种具有不同功能和特性的原理验证蛋白质的阵列。空腔具有与非常好的抗体相当的高亲和力（Kd～l nM）以及在单一氨基酸水平上的特异性。这项技术代表了我们研究新型蛋白质能力的突破，有可能解决将丰富的基因组信息转化为改进的药物发现的瓶颈。