Analysis of Lacrimal Gland by Laser Microdissection

激光显微切割泪腺分析

• 批准号: 6644709
• 负责人: JOHN L UBELS
• 金额: $ 5.63万
• 依托单位: VAN ANDEL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6644709
• 关键词: cell population study; epithelium; laboratory rat; lacrimal apparatus; lacrimal disorder; laser capture microdissection; morphometry; secretion; tear; technology /technique development; cell population study; epithelium; laboratory rat; lacrimal apparatus; lacrimal disorder; laser capture microdissection; morphometry; secretion; tear; technology /technique development

DESCRIPTION (provided by applicant): Malfunction of the lacrimal glands causes aqueous deficient dry eye. There is presently no treatment for lacrimal gland disease and progress towards development of effective therapy requires a thorough understanding of lacrimal function. The lacrimal gland is a tubulo-acinar gland with both acinar cells and ducts. The accessory lacrimal glands found in the palpebral and tarsal conjunctiva also produce aqueous tears. Little is known about the function of the lacrimal gland duct epithelium or accessory glands because of their small size and inaccessibility which makes it difficult to separate them from surrounding tissue. The purpose of the proposed study is to explore the feasibility of using laser capture microdissection to isolate and analyze these cell types. The specific aims of the project are to: 1) Sample rat exorbital lacrimal gland duct epithelial cells and accessory lacrimal gland acinar cells by laser capture microdissection, 2) compare gene expression among exorbital gland duct cells, exorbital gland acinar cells and accessory gland cells by gene microarray analysis, 3) identify selected genes that are differentially expressed in these cell types. The results of this study are expected to form the basis for further studies that will clarify the role of duct cells in the secretion and modification of lacrimal fluid and also lead to a better understanding of the role of accessory glands as compared to the main lacrimal gland in tear secretion.

描述（由申请人提供）：泪腺的故障导致水性缺乏干眼。目前没有治疗泪腺疾病的治疗方法，并且在开发有效疗法方面的进展需要彻底了解富富功能。泪腺是带有腺泡细胞和管道的微管腺腺。在pal骨和tarsal结膜中发现的辅助富集腺也会产生水性眼泪。由于其尺寸较小和无法访问，因此对泪腺上皮或辅助腺的功能知之甚少，这使得很难将它们与周围的组织分开。拟议的研究的目的是探索使用激光捕获显微解剖以分离和分析这些细胞类型的可行性。该项目的具体目的是：1）样品大鼠的卵形富腺导管上皮细胞和辅助富环腺腺泡细胞通过激光捕获微分辨率，2）比较exorbital腺管细胞中的基因表达，通过基因分析，在基因分析中鉴定了相同的基因分析，以表达型细胞，从而识别出exorbital腺细胞细胞和附属细胞。预计这项研究的结果将构成进一步研究的基础，这些研究将阐明管道细胞在泪液的分泌和修饰中的作用，并可以更好地理解与主要泪腺在泪液中相比的辅助腺的作用。