CORE--PHYSIOLOGY

核心--生理学

• 批准号: 6593879
• 负责人: LAUREN E COHN
• 金额: $ 16.54万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6593879
• 关键词: T lymphocyte; biomedical facility; bronchoconstrictors; bronchodilators; cytokine; genetically modified animals; laboratory mouse; respiratory function; respiratory hypersensitivity

DESCRIPTION (provided by applicant): The Physiology Core will provide technical expertise to the investigators in this program in the measurement of lung function in mice. This laboratory will provide assistance with experimental design, performance of pulmonary function testing and analyses of lung function measurements. This facility has been active for the past seven years during which time we have tested lung function in both anesthetized mice using direct measures of resistance and compliance and indirect measures of lung function by barometric whole body plethysmography to evaluate baseline and dose-responses to cholinergic, non-specific agonists. The studies have been performed on mice with different genetic backgrounds and mice with a range of genetic alterations, lung specific cytokine overexpression, cytokine and cytokine receptor gene deficient mice, and mice deficient in different transcription factors. These studies have taken advantage of multiple different models of airway inflammation, including inducible overexpression systems, antigen and hapten (isocyanate) immunized/sensitized mice and mice sensitized after adoptive transfer of in vitro generated T cells. Our past experience has provided us with data in each of these animals models defining the optimal age of the animals and timing for performing these studies. The aims of the Core are to 1) characterize alterations in baseline lung function and bronchoconstrictor response to cholinergic agonists in vivo; 2) determine the reversibility of altered lung function using inhaled bronchodilators. The Core will provide the facilities and expertise to perform direct and indirect measurements of lung function, administration of intravenous and aerosolized agonists and bronchodilators and kinetic experiments.

描述（由申请人提供）： 生理核心将为调查人员提供技术专长 该程序用于测量小鼠肺功能。这个实验室 将提供实验设计，肺部性能的帮助 肺功能测量的功能测试和分析。 在过去的七年中，该设施一直活跃在此期间 已经使用直接测量的两只麻醉小鼠的肺功能 通过气压的抗药性，依从性和间接度量 全身杂质的图表，以评估基线和剂量回答 胆碱能，非特异性激动剂。研究已经对小鼠进行 具有不同的遗传背景和小鼠具有一系列遗传 改变，肺特异性细胞因子过表达，细胞因子和细胞因子 受体基因缺乏小鼠，而小鼠缺乏不同的转录 因素。这些研究利用了多种不同模型的 气道炎症，包括可诱导的过表达系统，抗原和 触觉（异氰酸酯）免疫/敏化小鼠，而小鼠在敏化后敏化 体外产生的T细胞的过继转移。我们过去的经验有 在每个动物模型中为我们提供了定义最佳年龄的数据 动物和进行这些研究的时间。 核心的目的是1）表征基线肺的改变 对体内胆碱能激动剂的功能和支气管收缩反应； 2）确定使用吸入的肺功能改变的可逆性 支气管扩张剂。核心将为设施和专业知识提供 对肺功能进行直接和间接测量，给药 静脉和雾化激动剂和支气管扩张剂以及动力学 实验。