Core--RNA expression profiling

核心--RNA表达谱

• 批准号: 6659336
• 负责人: ALFRED LM BOTHWELL
• 金额: $ 17.75万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6659336
• 关键词: RNA; biomedical facility; blood vessels; gene expression; genetic markers; microarray technology

RNA Expression Profiling Core. A comprehensive analysis of RNA expression in distinct cell types has been revolutionized by the use of DNA microarray analysis. This methodology can assess the expression patterns of thousands of genes in quantitative terms with small amounts of cells or tissue. This analysis seeks to develop genetic markers for the models under study in each aim in the in vitro and in vivo analyses that form the basis of this proposal. Identification of such markers can then be used to facilitate the validation of the hypotheses of these models. The use of RNA markers should be a reliable method for defining cellular expression profiles. Once a set of markers has been identified these sequences can then be used by additional methods (e.g., in situ hybridization) to further test the hypotheses. Core E will work with investigators in Projects 1 and 2 to identify markers from cultured cells and from experimentally manipulated vascular tissues that can be compared with RNA prepared from human GA and control arteries in Core D.

RNA表达分析核心。通过使用DNA微阵列分析，对不同细胞类型的RNA表达进行了全面分析。该方法可以用少量的细胞或组织来评估数千种基因的表达模式。该分析旨在在体外和体内分析中为所研究模型开发遗传标记，从而构成该建议的基础。然后，可以使用此类标记的识别来促进这些模型假设的验证。 RNA标记的使用应该是定义细胞表达谱的可靠方法。一旦确定了一组标记，就可以通过其他方法（例如原位杂交）来进一步检验假设。核心E将与项目1和2中的研究人员合作，以鉴定来自培养细胞的标记以及可以将可与由人类GA制备的RNA进行比较的实验操纵的血管组织，并在核心D中进行对照动脉。