CORE--TRANSGENIC AND KNOCKOUT ANIMAL

核心——转基因和基因敲除动物

• 批准号: 6664445
• 负责人: GARY S JOHNSON
• 金额: $ 25.04万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-06 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6664445
• 关键词: biomedical facility; cryopreservation; embryo /fetus preservation; gene targeting; genetically modified animals; laboratory mouse

Description: (Applicant's Description) The Transgenic/Knockout Shared Core Resource is an expansion from the existing transgenic mouse core laboratory to include targeted gene disruption using homologous recombination in embryonic stem (ES) cells and the generation of "knockout" mice. The facility will also support the in vitro differentiation of ES cells. This core, which began operation in 1990, now consists of has an ES cell/targeted gene disruption facility and a microinjection laboratory. The core provides all the necessary services to support the generation, maintenance, breeding and preservation of transgenic and germ line positive knockout mice. Over the past four and one-half years, the transgenic component of this resource has served 25 different Cancer Center Investigators and generated 241 transgenic founders representing 44 constructs. The new ES cell/gene disruption facility has recently generated four germ line positive knockout mouse lines. In addition, the ES cell laboratory provides technical expertise in growth of ES cells and targeted gene disruption using homologous recombination and instruction of investigators in methods of in vitro ES cell differentiation. The core also provides embryo cryopreservation, strain rederivation, training of Cancer Center investigators, their graduate and postdoctoral students in transgenic technology, models and genetics. This component also provides colony management and maintenance, automated pedigree management, and breeding of all transgenic founders, knockout and experimental breeding colonies. The Transgenic/Knockout core presents a comprehensive service available to Cancer Center investigators for manipulation of the mouse genome. The primary goal is to maintain a state-of-the-art Transgenic/Knockout facility in the Cancer Center that is affordable and accessible to investigators for their studies in cancer biology.

描述：（申请人的描述）转基因/基因敲除共享核心 资源是从现有的转基因小鼠核心实验室扩展到 包括在胚胎中使用同源重组来破坏目标基因 干细胞（ES）和“基因敲除”小鼠的产生。该设施还将 支持ES细胞的体外分化。这个核心，始于 1990 年开始运营，现在包括 ES 细胞/靶向基因破坏 设施和显微注射实验室。核心提供了所有必要的 支持产生、维护、繁殖和保存的服务 转基因和种系阳性敲除小鼠。过去四年和 一年半的时间里，该资源的转基因成分已服务了 25 不同的癌症中心研究人员并产生了 241 名转基因创始人 代表 44 个结构。新的 ES 细胞/基因破坏设施 最近产生了四个种系阳性敲除小鼠系。此外， ES细胞实验室提供ES细胞生长方面的技术专业知识和 使用同源重组和指导进行靶向基因破坏 体外 ES 细胞分化方法的研究人员。核心还 提供胚胎冷冻保存、菌株再衍生、癌症训练 中心研究人员、他们的转基因研究生和博士后 技术、模型和遗传学。该组件还提供了菌落 管理和维护、自动化谱系管理以及所有物种的繁育 转基因创始人、淘汰和实验育种群体。这 转基因/基因敲除核心为癌症提供全面的服务 操纵小鼠基因组的中心研究人员。主要目标 是在癌症中心维持最先进的转基因/基因敲除设施 研究人员可以负担得起且可以使用的中心进行研究 癌症生物学。