CORE--FLOW CYTOMETRY

核心——流式细胞术

• 批准号: 6664425
• 负责人: Christopher Lee Reardon
• 金额: $ 25.04万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-06 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6664425
• 关键词: bioimaging /biomedical imaging; biomedical facility; cellular oncology; confocal scanning microscopy; enzyme linked immunosorbent assay; flow cytometry

Description: (Applicant's Description) The objective of the Flow Cytometry Core is to provide state-of-the-art flow cytometric analysis, cell-sorting services and immunology services to Cancer Center members. The Core laboratory consists of two flow cytometry facilities, one at the University of Colorado Health Sciences Center (UCHSC) directed by Christopher L. Reardon, PhD MD who also is overall director and the other facility at National Jewish Medical and Research Center (NJMRC) directed by John Cambier, PhD. A new source at UCHSC provides new immunological services other than flow cytometry. The presence of the Flow Cytometry Core at two sites provides Cancer Center members with convenient access to core facilities which have both technical expertise and multi-instrument capabilities in flow cytometry. In 1999, Dr. Reardon replaced Dr. Bunn as the director of this Core laboratory. Use of the Flow Cytometry Core at UCHSC is among the highest and most popular of all share cores and continues to increase. Most noted is its increase of use by 38% over the past 2 years, serving 68 different Cancer Center members over the last year. The services of the Core include analysis of cellular antigen expression, intracellular calcium and pH levels, DNA and RNA content, cell cycle kinetics, and assays of apoptosis. Single-cell cloning and sterile cell sorting is regularly done using 4-5 parameters, although both MoFlo sorters are capable of sorting on up to 9 different parameters. Confocal microscopy provides measurements of cell size by computer based micrometry, DNA/RNA content analysis, and ion movement analysis. New services include the assessment of natural killer cell, Fas-mediated and cell-mediated antigen-specific cytotoxicity with the non-radioactive euopium-chelate release assay which is measured by time-resolved fluorometry. Other new services using this technology are fluorescence-assisted phagocytosis and chemiluminescence measurements for oxidative bursts of granulocytes. Additional new services include ELISAs and T-cell hybridoma production for T cell immortalization, and cytokine assays based on ELISA, Golgi-immunostaining and elispot assays. The Core is certified by the College of American Pathologists (CAP) for analysis of clinical research trial specimens.

描述：（申请人的描述）流式细胞术的目的 核心是提供最先进的流式细胞术分析、细胞分选 为癌症中心成员提供服务和免疫学服务。核心 实验室由两个流式细胞仪设施组成，其中一个位于大学 科罗拉多健康科学中心 (UCHSC) 由 Christopher L. Reardon 领导， 医学博士，同时也是国家犹太医院其他设施的总主任 医学与研究中心 (NJMRC) 由 John Cambier 博士领导。一个新的 UHSC 的来源提供除流式细胞术以外的新免疫学服务。 两个地点的流式细胞术核心的存在为癌症中心提供了 会员可以方便地使用拥有技术的核心设施 流式细胞术方面的专业知识和多仪器能力。 1999 年，博士。 Reardon 取代 Bunn 博士成为这个核心实验室的主任。使用 UHSC 的流式细胞术核心是最高和最受欢迎的核心之一 共享核心并持续增加。最值得注意的是它的使用量增加 过去 2 年占 38%，为 68 名不同的癌症中心成员提供服务 去年。核心服务包括细胞抗原分析 表达、细胞内钙和 pH 水平、DNA 和 RNA 含量、细胞 循环动力学和细胞凋亡分析。单细胞克隆和无菌细胞 分拣通常使用 4-5 个参数完成，尽管两种 MoFlo 分拣机 能够对多达 9 个不同的参数进行排序。共焦显微镜 通过基于计算机的测微法、DNA/RNA 提供细胞尺寸测量 含量分析和离子运动分析。新服务包括 Fas 介导和细胞介导的自然杀伤细胞评估 非放射性铕螯合物释放的抗原特异性细胞毒性 通过时间分辨荧光测定法进行测定。其他新服务 使用该技术的是荧光辅助吞噬作用和 粒细胞氧化爆发的化学发光测量。 其他新服务包括 ELISA 和 T 细胞杂交瘤生产 细胞永生化以及基于 ELISA、高尔基体免疫染色的细胞因子测定 和酶联斑点测定。核心获得美国学院认证 病理学家 (CAP) 用于分析临床研究试验标本。