CORE--MORPHOLOGY

核心--形态学

• 批准号: 6563242
• 负责人: HIDEKAZU TSUKAMOTO
• 金额: $ 17.85万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563242
• 关键词: alcoholic liver cirrhosis; animal tissue; antibody; biomedical facility; cytokine; histology; immunocytochemistry; immunofluorescence technique; immunoperoxidase; nuclear factor kappa beta; pancreatitis; sectioning; tissue /cell preparation

The main objective of the Morphology Core is to support the Center Projects' pursuit for the Center's theme with high-quality and specialized morphologic services while achieving the net cost effectiveness. The types of services to be offered are classified into three categories: 1) standard staining of sections following paraffin embedding of tissue s(e.g. hematoxylin and eosin, sinus red for collagen); 2) special immunohistochemistry for frozen sections (e.g. inflammatory cells, cytokines, NF-kappaB); 3) image and morphometric analysis to provide standardized quantitative data. Samples which will be handled by the ore include liver (Research Project 1,2,3) and pancreas (Research Project 4) tissues, primary cultu4res of hepatocytes (Research Project 2), and isolated pancreatic acini (Pilot Project 1). Special staining procedures will be carried out on these samples after preparation of frozen sections, fixation, embedding, cutting, mounting on slides. In the case of cell culture, the cell will be permeabilized and immunostained. Immunofluorescent or immunoperoxidase staining will be performed using specific antibodies against cell constituents, cytokines, chemokines, and NF-kappaB. Quantitation of immunohistochemical staining will be performed by image analysis and/or densitometry. Blinded morphometric analysis for histologic assessment such as fat accumulation, necrosis, fibrosis will also be performed in a standardized fashion. Quality control is maintained at the proposed Core site, Anatomic Pathology Laboratory at Harbor-UCLA Medical Center, which is annually inspected and certified by the College of American Pathology and is under the directorship of the Core Director.

形态学核心的主要目标是通过高质量和专业的形态学服务支持中心项目对中心主题的追求，同时实现净成本效益。提供的服务类型分为三类：1）组织石蜡包埋后的标准切片染色（例如苏木精和曙红，胶原蛋白的窦红）； 2）冰冻切片特殊免疫组化（如炎症细胞、细胞因子、NF-kappaB）； 3）图像和形态测量分析提供标准化定量数据。矿石处理的样品包括肝脏（研究项目1、2、3）和胰腺（研究项目4）组织、肝细胞原代培养物（研究项目2）和分离的胰腺腺泡（试点项目1）。 在制备冰冻切片、固定、包埋、切割、安装在载玻片上后，将对这些样品进行特殊染色程序。在细胞培养的情况下，细胞将被透化和免疫染色。将使用针对细胞成分、细胞因子、趋化因子和 NF-κB 的特异性抗体进行免疫荧光或免疫过氧化物酶染色。免疫组织化学染色的定量将通过图像分析和/或光密度测定法进行。用于组织学评估（例如脂肪积累、坏死、纤维化）的盲态形态测量分析也将以标准化方式进行。质量控制由拟议的核心地点——加州大学洛杉矶分校港口医学中心的解剖病理学实验室进行，该实验室每年由美国病理学院进行检查和认证，并由核心主任领导。