Whole Animal ELISA for Liver Toxicity

整个动物 ELISA 检测肝脏毒性

• 批准号: 6547143
• 负责人: WEN L SENG
• 金额: $ 18.58万
• 依托单位: PHYLONIX PHARMACEUTICALS, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6547143
• 关键词: alternatives to animals in research; biomarker; drug adverse effect; drug screening /evaluation; enzyme linked immunosorbent assay; hepatotoxin; hybridomas; monoclonal antibody; technology /technique development; toxicant screening; zebrafish

DESCRIPTION (provided by applicant):The zebrafish, which has a morphological and molecular basis of tissue and organ development similar to that found in humans, is an ideal, vertebrate animal model for toxicity testing. In contrast to mice and rats, zebrafish are inexpensive to breed, undergo rapid embryogenesis and are easy to analyze because their embryos are transparent. Furthermore, drug delivery is simple and good dose responsiveness to toxicity has been observed. Currently zebrafish toxicity assays use lethality, embryo survival rate, behavior and microscopic examination of organ malformation as general parameters for assessing toxicity. However, for large-scale toxicity screening, qualitative visual inspection is not suitable. Using zebrafish embryos, this Phase I SBIR aims to develop a microplate-based ELISA assay to quantify liver toxicity. A rapid, quantitative assay for screening compounds for developmental organ toxicity will facilitate selection of lead compounds for further drug development. PROPOSED COMMERCIAL APPLICATIONS: By providing a rapid, quantitative, and inexpensive method for organ toxicity testing, the zebrafish assay will facilitate drug screening and toxicity testing in the pharmaceutical and chemical industries.

描述（由申请人提供）：斑马鱼具有与人类相似的组织和器官发育的形态和分子基础，是用于毒性测试的理想脊椎动物模型。与小鼠和大鼠相比，斑马鱼的繁殖成本低廉，胚胎发生速度快，并且由于胚胎是透明的而易于分析。此外，药物递送简单并且对毒性具有良好的剂量反应性。目前斑马鱼毒性测定使用致死率、胚胎存活率、行为和器官畸形的显微镜检查作为评估毒性的一般参数。然而，对于大规模毒性筛查，定性目视检查并不适合。该 I 期 SBIR 旨在使用斑马鱼胚胎开发一种基于微孔板的 ELISA 测定法来量化肝脏毒性。用于筛选化合物的发育器官毒性的快速定量测定将有助于选择先导化合物以用于进一步的药物开发。 拟议的商业应用：通过提供一种快速、定量且廉价的器官毒性测试方法，斑马鱼测定将促进制药和化学工业中的药物筛选和毒性测试。