Output of the Circadian Clock

昼夜节律时钟的输出

• 批准号: 6539269
• 负责人: HERMAN WIJNEN
• 金额: $ 4.42万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6539269
• 关键词: Drosophilidae; RNase protection assay; animal genetic material tag; behavioral /social science research tag; behavioral genetics; biological clocks; circadian rhythms; data collection methodology /evaluation; gene expression; genetically modified animals; in situ hybridization; microarray technology; northern blottings; nucleic acid probes; polymerase chain reaction; psychic activity level; rest; sleep; transcription factor; wakefulness

DESCRIPTION (provided by applicant): Circadian (-24 hour) rhythms that are controlled by circadian clocks are found in the physiology and behavior of many organisms including humans and fruitflies. In mammals the sleep-wake cycle is the most obvious example of a circadian rhythm that can have important effects on health, but the regulation of body temperature, heartbeat, blood pressure, endocrine functions, renal activity, and liver metabolism have also been shown to be under circadian regulation. Circadian clocks generate oscillating patterns of transcription that control both the rhythm of the clocks themselves and the output that they produce. It is the goal of this research project to first test on a genome-wide scale which transcripts in the head of adult fruitflies are regulated by circadian clocks and then identify a subset of these transcripts that are involved in the regulation of the rest-activity cycle. DNA microarrays will be used to perform the expression analyses; locomotor activity assays will serve as a phenotypic measure for the rest-activity cycle; and a combination of genetic and molecular techniques will be employed in the functional analysis of candidate regulators of the rest-activity cycle. The comprehensive description of the clock controlled transcripts in Drosophila heads will provide a valuable resource for all future studies on circadian rhythms, whereas the discovery of new mediators of the rest-activity cycle will promote a better understanding of the molecular mechanisms underlying both the rest-activity cycle in fruitfiles and the analogous sleep-wake cycle in mammals.

描述（由申请人提供）：昼夜节律的节奏 在许多人的生理和行为中发现了由昼夜节律控制的 包括人类和果蝇在内的生物。在哺乳动物中，睡眠觉醒周期为 昼夜节律最明显的例子，可以产生重要的影响 关于健康，但调节体温，心跳，血压， 还显示了内分泌功能，肾脏活性和肝代谢 受到昼夜节律的监管。昼夜节律产生振荡 控制时钟本身的节奏的转录模式 以及它们产生的输出。这是该研究项目的目标 在全基因组量表上进行的首次测试 果蝇由昼夜节律调节，然后确定 这些涉及休息活性的成绩单 循环。 DNA微阵列将用于执行表达分析。 运动活性测定将作为表型措施 休息活性周期；遗传和分子技术的结合将 被用于候选监管机构的功能分析 静止活性周期。时钟控制的全面描述 果蝇头部的成绩单将为所有未来提供宝贵的资源 关于昼夜节律的研究，而发现了新的调解人 静止活性周期将促进对分子的更好理解 水果文件中的静止循环和 哺乳动物中的类似睡眠效果周期。