CAMPYLOBACTER

弯曲杆菌

• 批准号: 6311619
• 负责人: GUILLERMO RUIZ-PALACIOS
• 金额: $ 13.13万
• 依托单位: EASTERN VIRGINIA MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6311619
• 关键词: CHO cells; Helicobacter; Mexico; bacteria infection mechanism; breast feeding; cell adhesion; clinical research; clinical trials; diarrhea; gastrointestinal epithelium; genetically modified animals; glycolipids; glycoproteins; hexosyltransferase; human milk; human subject; infant human (0-1 year); laboratory mouse; nutrition related tag; passive immunization; receptor binding; virulence

Campylobacter is one of the most common cause of diarrhea worldwide. Human milk protects young infants against Campylobacter diarrhea by several factors including fucosylated oligosaccharides. However, there is a lack of understanding of the chemical structure of these components and their mechanism of interaction with Campylobacter virulence traits associated with attachment to gut mucosa. The specific aims of this project are: 1) define the virulence markers of Campylobacter associated with attachment to epithelial cells; 2) characterize and purify milk glycoconjugates that inhibit Campylobacter infection; 3) assess passive protection in breast- fed children and experimentally in transgenic mice carrying a human fucoslytransferase gene expressed mainly in mammary tissue; 4) develop strategies for large-scale synthesis of fucosylated glycoconjugates active against Campylobacter infection; and 5) conduct clinical trials on safety and protection of fucosylated glycoconjugates against Campylobacter diarrhea. The long-term goal of this project is to develop methods for prevention and treatment of Campylobacter infection through understanding at the cellular and molecular level the mechanism of protection conferred by human milk against Campylobacter infection.

弯曲杆菌是全世界腹泻最常见的原因之一。人类 牛奶可以通过多种方式保护小婴儿免受弯曲菌腹泻的影响 因素包括岩藻糖基化寡糖。然而，还存在不足 了解这些成分的化学结构及其作用 与弯曲杆菌毒力特性相关的相互作用机制 与肠粘膜附着。该项目的具体目标是：1） 定义与附着相关的弯曲杆菌的毒力标记 上皮细胞； 2) 表征和纯化乳糖复合物 抑制弯曲杆菌感染； 3）评估乳房的被动保护- 喂养儿童并在携带人类的转基因小鼠中进行实验 岩藻糖基转移酶基因主要在乳腺组织中表达； 4）开发 大规模合成岩藻糖基化糖复合物活性的策略 对抗弯曲杆菌感染； 5）进行安全性临床试验 和岩藻糖基化糖复合物对弯曲杆菌的保护 腹泻。该项目的长期目标是开发方法 了解弯曲杆菌感染的预防和治疗 在细胞和分子水平上赋予的保护机制 通过母乳对抗弯曲杆菌感染。