MOUSE SYNAPTIC VESICULAR MONAMINE TRANSPORTER

小鼠突触囊泡单胺转运蛋白

• 批准号: 6103871
• 负责人: George Richard Uhl
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON DRUG ABUSE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6103871
• 关键词: amphetamines; animal genetic material tag; complementary DNA; dopamine; dopamine transporter; embryonic stem cell; gene deletion mutation; genetic regulatory element; laboratory mouse; methylphenyltetrahydropyridine; molecular cloning; neurotoxicology; neurotoxins; neurotransmitter transport; nucleic acid sequence; synaptic vesicles; transcription factor; transfection /expression vector

Activities of the principal brain vesicular monamine transporter, VMAT2, are key to understanding the cellular compartmentalization of monoamines that may play a key role in modulating the actions and neurotoxicities induced by amphetamine and by each of the toxins that selectively kills dopaminergic neurons to provide the best current models of Parkinson's disease. In this FY, workers in this Branch described the properties of knockout mice with deletions of the VMAT2 gene, as well as the structure of this gene itself in mice and humans. VMAT2 deficient heterozygote mice show selective alterations in their responses to cocaine and amphetamine, as well as differences in MPTP toxicities. These mice substantially enhance our understanding of mechanisms of psychostimulant and dopaminergic neurotoxin action.

主要脑泡单胺的活性 转运蛋白 VMAT2 是了解细胞的关键 单胺的区室化可能发挥关键作用 调节安非他明引起的作用和神经毒性 以及选择性杀死多巴胺能神经元的每种毒素 提供当前最好的帕金森病模型。在这个 FY，该部门的工作人员描述了淘汰赛的特性 缺失 VMAT2 基因的小鼠及其结构 这个基因本身存在于小鼠和人类中。 VMAT2 缺陷杂合子 小鼠对可卡因的反应表现出选择性改变 安非他命，以及 MPTP 毒性的差异。这些老鼠 大大增强我们对机制的理解 精神兴奋和多巴胺能神经毒素作用。