IN VITRO SCREENING OF CHEMOPREVENTIVE AGENTS THAT INHIBI

抑制化学预防剂的体外筛选

• 批准号: 6352449
• 负责人: NITIN T TELANG
• 金额: $ 35万
• 依托单位: STRANG CANCER PREVENTION CENTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6352449
• 关键词: antigen presenting cell; biomarker; cancer prevention; cell line; cell transformation; chemoprevention; colorectal neoplasms; drug screening /evaluation; laboratory mouse; tissue /cell culture

The efficacy of chemopreventive agents is tested against clinically-normal colon epithelial cells derived from mice predisposed to colon cancer. The primary cell cultures are expanded in culture and assayed for surrogate biomarkers prior to and following their spontaneous immortalization in the presence or absence of chemopreventive agents. The mutant mice used are as follows: MIN, MLH1, APC/MLH1, and SMAD4.

化学预防剂的功效是针对来自易患结肠癌的小鼠的临床正常结肠上皮细胞进行测试的。原代细胞培养物在培养物中扩增，并在其自发永生化之前和之后在存在或不存在化学预防剂的情况下测定替代生物标志物。使用的突变小鼠如下：MIN、MLH1、APC/MLH1 和 SMAD4。