Characterization of the Vibrio harveyi CgtA protein

哈维氏弧菌 CgtA 蛋白的表征

• 批准号: 6548421
• 负责人: Janine R. Maddock
• 金额: $ 4.03万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6548421
• 关键词: Caulobacter; DNA repair; DNA replication; Escherichia coli; Vibrio; bacterial proteins; cell cycle; cell cycle proteins; cooperative study; gene expression; genetic regulation; guanine nucleotide binding protein; hydrolysis; mutant; phosphorylation; protein localization; protein protein interaction; protein structure function; ribosomes; site directed mutagenesis

DESCRIPTION (provided by applicant) GTP-binding proteins play crucial roles in the regulation of fundamental processes in cells of all living organisms. In fact, small monomeric GTP-binding proteins have been found in every organism examined thus far. In eukaryotic cells, these proteins are involved in a number of essential processes, such as signal transduction, protein synthesis and translocation or cell cycle regulation. However, perhaps surprisingly, relatively little information is currently available about roles of GTP-binding proteins in prokaryotes. A subfamily of small GTP-binding proteins, including products of obg and cgtA genes from Bacillus subtilis and Caulobacter crescentus respectively, was discovered recently in bacteria. Members of this subfamily are present in diverse organisms ranging from bacteria to humans. On the other hand, functions of these proteins in regulation of cellular processes are largely unknown. Genes coding for these proteins are essential in almost all bacteria investigated thus far. However a viable Vibrio harveyi insertional mutant in the cgtA gene was described recently by us. Therefore, this mutant gives a unique opportunity to study functions of a member of the subfamily of Obg-like proteins. The goal of this work is to investigate roles of the CgtA protein in the regulation of cellular processes. Our preliminary experiments suggest that the cgtA gene product may be involved in regulation of crucial chromosome functions like synchronization of DNA replication initiation, chromosome partition and DNA repair. We plan to perfom both gentic and biochemical studies to learn about processes and mechanisms controlled by CgtA and about biochemical functions of this protein. Since there is a strong similarity between CgtA-like proteins in diverse organisms, including humans, we believe that studies planned by us and described is this application are of general biological significance.

描述（由申请人提供）GTP结合蛋白在调节所有生物体细胞的基本过程中起着至关重要的作用。实际上，在迄今为止检查的每个生物体中都发现了小的单体GTP结合蛋白。在真核细胞中，这些蛋白质参与了许多基本过程，例如信号转导，蛋白质合成和易位或细胞周期调节。但是，可能令人惊讶的是，目前有关GTP结合蛋白在原核生物中的作用的信息相对较少。最近在细菌中发现了小型GTP结合蛋白的亚家族，包括枯草芽孢杆菌和花椰菜新月形的CGTA基因的产物。该亚家族的成员存在于从细菌到人类的各种生物中。另一方面，这些蛋白质在调节细胞过程中的功能在很大程度上尚不清楚。这些蛋白质编码的基因在迄今为止研究的几乎所有细菌中都是必不可少的。然而，我们最近描述了CGTA基因中一个可行的颤音harveyi插入突变体。因此，该突变体提供了一个独特的机会来研究obg样蛋白亚科成员的功能。这项工作的目的是研究CGTA蛋白在细胞过程调节中的作用。我们的初步实验表明，CGTA基因产物可能参与了关键染色体功能的调节，例如DNA复制起始，染色体分区和DNA修复的同步。我们计划培养绅士和生化研究，以了解CGTA控制的过程和机制以及该蛋白质的生化功能。由于包括人类在内的各种生物中的CGTA样蛋白之间存在很强的相似性，我们认为我们和描述的研究是这种应用具有一般的生物学意义。