Composite Supports for High Throughput Chromatography

高通量色谱复合支撑物

• 批准号: 6529837
• 负责人: RICHARD F HAMMEN
• 金额: $ 33.04万
• 依托单位: CHELATECH, INC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-15 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6529837
• 关键词: biomaterial development /preparation; carboxylate; chemical synthesis; chromatography; crosslink; high performance liquid chromatography; ion exchange chromatography; nucleic acid chemical synthesis; polymers; protein purification; proteomics; silicon compounds; technology /technique development

DESCRIPTION: (provide by applicant) Research and drug development in the pharmaceutical industry rely heavily upon separation science, particularly in this era of High Throughput genomic and proteomic analyses. Chromatographic methods used today separate proteins from other molecules by interactions with a solid porous support Solutes and solvent flowing through a chromatographic column of porous media prefer the path of least resistance and flow around the particles, rather than the tortuous pathway through the narrow pores where the functional chemistry is located. This leads to slow adsorption-desorption kinetics, band spreading, and loss of resolution. This Phase I Research was successful in literally turning this problem inside out, by synthesizing composite materials consisting of nonporous particles with "spider webs" of functional ligands crossing the interstitial spaces between the particles. The Specific Aims of this Research are to synthesize a series of composite chromatography columns with functional groups immobilized by extended tethers that place the functional molecule thousands of Angstroms from the surface of the solid support. Prototype products will be developed for the High Throughput Chromatography of a variety of proteins in both a column format and in a parallel plate format that will perform separations in a few seconds. PROPOSED COMMERCIAL APPLICATION: The composite materials developed in this Research will find commercial use for High Throughput Chromatographic separations and bioassays. Corporate partners will assist ChelaTech in evaluating the performance and the market competitiveness of the prototype products developed in Phase II. The sale and deployment of these products to the pharmaceutical and healthcare research industries will enable cost reductions, enhanced accuracy, and productivity increases in research to cure human disease.

描述：（由申请人提供）研究和药物开发 制药行业严重依赖分离科学，特别是在 这个高通量基因组和蛋白质组分析的时代。色谱法 当今使用的方法通过与蛋白质的相互作用将蛋白质与其他分子分离 固体多孔载体 溶质和溶剂流经色谱 多孔介质柱更喜欢周围阻力和流动最小的路径 颗粒，而不是通过狭窄孔隙的曲折路径 功能化学位于。这导致缓慢的吸附-解吸 动力学、谱带展宽和分辨率损失。这一阶段的研究是 通过综合，成功地彻底解决了这个问题 由具有“蜘蛛网”的无孔颗粒组成的复合材料 功能配体穿过颗粒之间的间隙空间。这 本研究的具体目标是合成一系列复合材料 具有通过延长系绳固定的官能团的色谱柱 将功能分子放置在距表面数千埃的位置 的坚实支持。原型产品将针对高吞吐量而开发 各种蛋白质的色谱分析，包括柱形式和柱形式 平行板格式，可在几秒钟内完成分离。 拟议的商业应用： 本研究开发的复合材料将在高通量色谱分离和生物测定中找到商业用途。企业合作伙伴将协助ChelaTech评估二期开发的原型产品的性能和市场竞争力。这些产品向制药和医疗保健研究行业的销售和部署将有助于降低人类疾病研究的成本、提高准确性和生产力。