STRESS RESPONSIVE GENES AND LONGEVITY IN DROSOPHILA

果蝇的应激反应基因和长寿

• 批准号: 6509498
• 负责人: HORNG-DAR WANG
• 金额: $ 4.81万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6509498
• 关键词: Drosophilidae; complementary DNA; genetic library; heat stimulus; longevity; mutant; northern blottings; paraquat; plasmids; starvation; stress proteins; subtraction hybridization

The purpose of the proposal is to find multiple-stress-responsive genes from paraquat, starvation, and heat-treated Drosophila to study their effects on longevity. By using subtractive hybridization and cross hybridization, multiple-stress-responsive cDNA will be identified. Northern blot will be used to confirm the positive cDNA clones. The corresponding gene of each cDNA clone will be searched and cloned. The ability of each multiple- stress-responsive gene to resist stresses and extend longevity in Drosophila will be tested. A genetic screen of mutated flies for stress-resistance will find longevity flies and identify the longevity genes. The discovery of the new genes to increase longevity will help us to better understand the mechanisms of extended life-span.

该提案的目的是从帕拉夸酸，饥饿和热处理果蝇中找到多种压力响应的基因，以研究其对寿命的影响。 通过使用减法杂交和跨杂交，将确定多重应答cDNA。 北印迹将用于确认阳性cDNA克隆。 每个cDNA克隆的相应基因将被搜索和克隆。 每个多应力响应基因抵抗应力和延长果蝇中寿命的能力。 突变蝇的遗传筛选以抗压力抗性，将发现寿命苍蝇并识别寿命基因。 发现新基因以增加寿命将有助于我们更好地了解扩展寿命的机制。