Core--Microbiology Facility

核心--微生物设施

• 批准号: 6345958
• 负责人: Sharon L. Hillier
• 金额: $ 3.57万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6345958
• 关键词: Bacteroides; Chlamydia trachomatis; Lactobacillus; Mycoplasma; Mycoplasmatales; Neisseria gonorrhoeae; Staphylococcus; Streptococcus; Trichomonas vaginalis; biomedical facility; cooperative study; microorganism culture; polymerase chain reaction; sexually transmitted diseases; yeasts

The objective of Microbiology Core is to provide support for Project 1 (Microbicidal Lactobacilli for Prevention of Genital Infection), project 3 (Trichomonas vaginalis and SLPI Mucosal Defenses) and project 4 (Iron- regulated Proteins and Gonococcal Infection. The core laboratory will provide cervical cultures for Neisseria gonorrhoeae, vaginal cultures for Trichomonas vaginalis, and evaluation for Gram-strained vaginal smears for bacterial vaginosis. Cultures of vaginal fluid will be evaluated for lactobacilli (H2O2+ and H2O2-), Mycoplasma hominis, Ureaplasma urealyticum, obligately anaerobic gram negative rods including Prevotella, Prophyromonas and Bacteroides, Gardnerella vaginalis, yeast, staphylococci, streptococci and coliforms. Chlamydia trachomatis will be detected by PCR within the clinical laboratory at Magee-Womens Hospitals, but the Microbiology Core personnel will be responsible for delivery of patient specimens to the clinical laboratory for testing. The microbiology core will assist project leaders in developing specimen collection and transport protocols, will provide appropriate specimen collection media to the clinical core sites, work with the biostatistical- epidemiology core on coding and processing the microbiologic data, and provide assistance in evaluation of microbiologic data for interim reports and manuscripts.

微生物学核心的目标是为项目1（预防生殖器感染的杀菌乳杆菌）、项目3（阴道毛滴虫和SLPI粘膜防御）和项目4（铁调节蛋白和淋球菌感染）提供支持。核心实验室将提供宫颈淋病奈瑟菌培养、阴道毛滴虫阴道培养以及评估细菌性阴道病的革兰氏菌阴道涂片培养物将评估乳酸杆菌（H2O2+ 和 H2O2-）、人型支原体、解脲支原体、专性厌氧革兰氏阴性杆菌（包括普雷沃氏菌、Prophyromonas 和 Bacteroides、Gardnerella vaginalis、酵母菌、葡萄球菌）。链球菌沙眼衣原体将在 Magee-Womens 医院的临床实验室内通过 PCR 进行检测，但微生物学核心人员将负责将患者标本运送到临床实验室进行检测。微生物学核心人员将协助项目负责人开发标本。收集和运输协议，将为临床核心站点提供适当的标本收集介质，与生物统计流行病学核心合作编码和处理微生物数据，并为评估微生物学数据提供帮助中期报告和手稿的数据。