Identifying Genes Involved in Neuronal Differentiation

鉴定参与神经元分化的基因

• 批准号: 6529745
• 负责人: BIN CHEN
• 金额: $ 4.62万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6529745
• 关键词: developmental genetics; developmental neurobiology; gene induction /repression; gene targeting; genetically modified animals; laboratory mouse; microarray technology; neurogenesis; neurogenetics; subtraction hybridization; transcription factor; visual cortex

DESCRIPTION (provided by applicant): The mammalian cerebral cortex is a layered structure. I am interested in the molecules that regulate development of cortical layers. The first specific aim is to identify genes that are critical in the formation of proper axonal projection patterns for neurons within a specific cortical layer, layer 5. Layer 5 neurons initially extend axonal branches to many subcortical areas. Later the exuberant branches are selectively pruned so that a proper area-specific projection pattern is formed. The homeodomain transcription factor, Otx1, is required for the pruning of exuberant axonal branches. However nothing is known about the target genes of Otx l or about the mechanisms of axonal pruning. I propose to identify genes that act downstream of Otx1 to regulate axonal refinement using cDNA microarray technology. The second aim is to isolate and study genes which regulate the initial laminar fate determination or early stages of laminar differentiation. I have finished an in situ hybridization screening and identified twelve novel as well as known genes that demonstrate layer-specific expression patterns. Experiments are proposed to study their potential functions in laminar fate determination and/or early stages of layer differentiation.

描述（由申请人提供）：哺乳动物脑皮质是一个分层 结构。我对调节发展的分子感兴趣 皮质层。 第一个具体目的是识别在形成中至关重要的基因 特定皮质内神经元的适当轴突投影模式 层，第5层。第5层神经元最初将轴突分支扩展到许多 皮质下区域。后来，繁殖的分支被选择性地修剪 形成了适当的特定区域投影模式。同源域 转录因子OTX1是繁殖轴突修剪所必需的 分支。但是，关于OTX L或有关的目标基因或有关 轴突修剪的机制。我建议识别下游作用的基因 使用cDNA微阵列技术调节轴突改进的OTX1。 第二个目的是分离和研究调节初始层流的基因 命运确定或层流分化的早期阶段。我已经完成了 原位杂交筛选并确定了十二本小说并已知 证明层特异性表达模式的基因。实验是 提议研究其在层流命运确定中的潜在功能 和/或层分化的早期阶段。