CORE--ANIMAL MODELS AND IMMUNOLOGY LAB

核心--动物模型与免疫实验室

• 批准号: 6325715
• 负责人: Jonathan S Bromberg
• 金额: $ 15.76万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6325715
• 关键词: Adenoviridae; SCID mouse; aging; animal breeding; animal care; biomedical facility; cell mediated lymphocytolysis test; cellular immunity; disease /disorder model; dystrophin; gene therapy; genetically modified animals; humoral immunity; muscular dystrophy; striated muscles; transfection /expression vector

The central purpose of the Animal Models Core is to provide the affiliated investigators with the appropriate genotype and strain of rodent for the proposed studies, and to provide a range of immunological assays on animals inject with adenoviral vectors. The Animal Models Core is specifically dedicated to investigations of novel strategies of gene delivery to aged or dystrophic striated muscle, as well as mechanistic based evaluations of the effects of genetic modification of dystrophin on muscle cell mechanical properties in young and aged animals. All rodents will be raised and maintained under specific-pathogen-free conditions at the University of Michigan AAALAC-accredited animal facility. In addition to maintaining stocks of animals for testing, the core will conduct a detailed life span analysis of the dystrophic mdx mouse. This core lab will also handle all the immunology required for analysis of adenoviral vector development. A goal of our new vector design is to develop an adenoviral based vector that does not trigger a strong immune response in host animals, particularly a cytotoxic T-cell mediated response. Animals that are injected with virus will be used not only to assess the longevity and functional consequences of gene expression, but will also be tested for potential immune responses against the virus and the transgene being delivered. This analysis will involve CTL-assays against adenoviral and transgene proteins, as well as B cell mediated humoral immune responses against the same proteins.

动物模型核心的中心目的是提供附属的 具有适当基因型和啮齿动物品系的研究人员 提出的研究，并提供一系列免疫学测定 动物注射腺病毒载体。动物模型的核心是 专门致力于基因新策略的研究 递送至老化或营养不良的横纹肌，以及机械性 基于抗肌营养不良蛋白基因修饰影响的评估 年轻和老年动物的肌肉细胞机械特性。所有啮齿动物 将在无特定病原体的条件下饲养和维持 密歇根大学 AAALAC 认证的动物设施。此外 为了维持用于测试的动物库存，核心将进行 营养不良 mdx 小鼠的详细寿命分析。 该核心实验室还将处理分析所需的所有免疫学 腺病毒载体的开发。我们新矢量设计的目标是 开发一种基于腺病毒的载体，不会触发强烈的免疫 宿主动物的反应，特别是细胞毒性 T 细胞介导的反应 回复。注射病毒的动物不仅会被用来 评估基因表达的寿命和功能后果，但是 还将测试针对病毒的潜在免疫反应， 正在传递的转基因。该分析将涉及 CTL 检测 对抗腺病毒和转基因蛋白，以及 B 细胞介导的 针对相同蛋白质的体液免疫反应。