Median Preoptic Nucleus and the Control of Sleep

正中视前核和睡眠的控制

• 批准号: 6430418
• 负责人: Ronald Szymusiak
• 金额: $ 22.61万
• 依托单位: SEPULVEDA RESEARCH CORPORATION
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6430418
• 关键词: angiotensin II; blood osmolarity; body temperature regulation; brain electrical activity; cell population study; computer data analysis; electroencephalography; electromyography; fos protein; gamma aminobutyrate; gene expression; glutamate decarboxylase; immunocytochemistry; laboratory rat; microelectrodes; neuroanatomy; neurochemistry; neurogenetics; neurons; neuroregulation; polygraphy; preoptic areas; psychic activity level; saline; sleep regulatory center

A role for the preoptic area (POA) of the hypothalamus in the control of sleep is supported by the results of lesion, stimulation and neuronal recording experiments. The precise location of putative sleep-regulatory cell groups within the POA is incompletely understood, but recent progress has been made through application of the c-fos protein immunoreactivity (IR) method. A localized group of neurons in the ventrolateral POA (vlPOA) that exhibit Fos-IR during sleep has been described in rat. vlPOA neurons have been shown to project to monoaminergic arousal systems in the posterior hypothalamus and brainstem and to contain the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). Neuronal recording studies confirmed the presence of neurons with sleep-related discharge patterns within the vlPOA. The goal of proposed work is to characterize the anatomy and physiology of a second POA region where neurons exhibiting seep- related Fos-IR have been localized, the median preoptic nucleus (MnPN). Proposed experiments will answer the following questions: 1) Does the MnPN contain a high concentration of neurons with sleep-related discharge patterns, like that previously found in the vlPOA? 2) Are MnPN neurons that exhibit sleep-related Fos-IR also GABAergic? 3) Do MnPN neurons that exhibit sleep-related Fos-IR project to arousal systems in the magnocellular basal forebrain, the perifornical region of the lateral hypothalamus, the dorsal raphe nucleus and the dorsolateral pons? 4) Are a subset of MnPN neurons with sleep- related discharge also thermosensitive, as has been previously shown for vlPOA neurons? 5) Are MnPN neurons that are involved in the control of body fluid homeostasis a separate population from sleep-related MnPN neurons? Proposed work will examine in depth the anatomical, neurochemical, and electrophysiological features of an important POA neuronal group, the MnPN. Planned experiments will contribute to a more complete understanding of the role of hypothalamic mechanisms in normal and disordered sleep and arousal states.

病变，刺激和神经元记录实验的结果支持下丘脑控制的作用（POA）在睡眠控制中的作用。 POA中推定的睡眠调节细胞组的确切位置尚不完全理解，但是通过应用C-FOS蛋白免疫反应性（IR）方法取得了最新的进展。 在大鼠中，已经描述了在睡眠期间表现出FOS-IR的腹侧POA（VLPOA）中的局部神经元组。 已显示VLPOA神经元在下丘脑和脑干中投射到单胺能唤醒系统上，并包含抑制性神经递质γ-氨基丁酸（GABA）。 神经元记录研究证实了在VLPOA中存在与睡眠相关的放电模式的神经元。拟议工作的目的是表征第二个POA区域的解剖学和生理学，在该区域中，神经元表现出相关的FOS-IR已定位为中位数前核（MNPN）。 提出的实验将回答以下问题：1）MNPN是否包含具有与睡眠相关的放电模式的高浓度的神经元，就像以前在VLPOA中发现的神经元吗？ 2）是否表现出与睡眠相关的FOS-IR的MNPN神经元也有GABA能吗？ 3）表现出与睡眠相关的FOS-IR的MNPN神经元是否向大细胞基础前脑，下丘脑外侧下丘脑，背侧raphe核和背外侧Pons的垂体区域发射唤醒系统？ 4）像先前对VLPOA神经元所示的具有睡眠相关放电的MNPN神经元的子集也是热敏的吗？ 5）与与睡眠相关的MNPN神经元不同的MNPN神经元是否参与了体液稳态的控制？拟议的工作将深入研究重要的POA神经元组MNPN的解剖学，神经化学和电生理特征。 计划的实验将有助于更完整地了解下丘脑机制在正常和无序的睡眠和唤醒状态中的作用。