CORE--IN VITRO, IN VIVO ASSAY SYSTEMS

核心——体外、体内检测系统

• 批准号: 6587619
• 负责人: MICHAEL C SCHMALE
• 金额: $ 7.35万
• 依托单位: UNIVERSITY OF MIAMI CORAL GABLES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-22 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6587619
• 关键词: animal breeding; aquatic biology; aquatic organism; biomedical facility; electron microscopy; environmental toxicology; fish; genetic manipulation; genetically modified animals; marine toxins; tissue /cell culture; water environment; zebrafish

Description (provided by applicant): The emphasis of the In vitro and In vivo Fish Culture Core is to provide reagents, expert advice and actual services for investigators doing experiments with fish cell cultures or live fish of several species. Many of the ongoing and planned research projects of the Center utilize cultured cells for morphological, physiological, electrophysiological and biochemical studies. This Core will provide all resources necessary for set-up, maintenance and analysis of cell cultures from aquatic fishes (and invertebrates). This will include assistance in developing new techniques for specialized needs as well as application of routine procedures for cell maintenance and manipulation. The technician in this Core will also be available to set-up and maintain cultures, when necessary. A variety of damselfish cell lines and primary cultures and zebrafish primary cultures will be continuously available to investigators. Any of the fish cell lines maintained by the American Type Culture Collection will also be obtained and maintained as needed by the technician in this Core. Within this Core three whole animal facilities, the damselfish carcinogenesis and transgenic zebrafish laboratories and the controlled exposure system, will also be made available to Center investigators. In addition, this Core will act as an intermediary to obtain animals, holding tanks and animal care services from the experimental fish hatchery on the RSMAS campus. This function was previously performed by Core 6, which will be dropped in the proposed renewal program. This reallocation of resources is based on experience with actual usage levels of Core 6 during the past 5 years.

描述（由申请人提供）：体外和体内的重点 鱼类文化核心将提供试剂，专家建议和实际服务 用于研究鱼细胞培养物或活鱼的研究人员 几个物种。许多正在进行的和计划的研究项目 中心利用培养的细胞进行形态学，生理， 电生理学和生化研究。这个核心将提供全部 从 水生鱼类（和无脊椎动物）。这将包括协助 为专业需求开发新技术以及应用 用于维护和操纵的常规程序。技术人员在 该核心也将用于设置和维护文化 必要的。各种各样的狂潮细胞系和原发性培养物以及 斑马鱼原发性文化将不断为研究人员提供。 美国类型培养物收藏维护的任何鱼类细胞系 技术人员还将根据该核心获得和维护并维护。 在这个核心三个动物设施中，大坝癌发生 以及转基因斑马鱼实验室和受控的暴露系统，将 也可以向中心调查人员提供。 此外，该核心将充当获取动物的中介 来自实验性鱼类孵化场的坦克和动物护理服务 RSMAS校园。此功能以前是由Core 6执行的，这将是 丢弃了拟议的更新计划。资源的重新分配是 根据过去5的实际使用级别的实际使用水平的经验 年。