STRUCTURAL STUDIES OF THE BACTERIORHODOPSIN PHOTOCYCLE

细菌视紫红质光循环的结构研究

• 批准号: 6526218
• 负责人: HARTMUT LUECKE
• 金额: $ 22.16万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6526218
• 关键词: X ray crystallography; bacteriorhodopsins; chloride ion; conformation; hydrogen transport; ion transport; photochemistry; protein structure function; retinaldehyde; structural biology

DESCRIPTION (Adapted from abstract): Bacteriorhodopsin is a light-driven proton pump that has several well-characterized intermediates in its photocycle. The general aim of this proposal is to define the mechanism of proton transport in bacteriorhodopsin by mapping the conformational changes during the photocycle in atomic detail. The specific aims are 1) to determine the high resolution structure of light-adapted (ground state) bacteriorhodopsin, 2) determine the structure of the M intermediate by solving the structure of D96N bacteriorhodopsin 3) determine the crystal structure of the K intermediate, 4) solve the crystal structure of the L intermediate and 5) solve the structure of a chloride pump, either halorhodopsin, or D85S bacteriorhodopsin, which functions as a chloride pump.

描述（根据摘要改编）：细菌紫红质是一种轻驱动质子 其光圈中具有多个特征良好的中间体的泵。这 该提议的一般目的是定义质子运输机制 通过映射光循环期间的构象变化， 原子细节。具体目的是1）确定高分辨率 灯光适应的结构（基态）细菌紫红素，2）确定 M中间的M中间结构通过求解D96N的结构 细菌近红杉3）确定K中间体的晶体结构，4） 解决L中间体的晶体结构，5）解决 氯化泵，甲状腺淡蛋白或D85S细菌紫红素 用作氯化泵。

项目成果

Crystallization, X-ray diffraction analysis and SIRAS/molecular-replacenent phasing of three crystal forms of Anabaena sensory rhodopsin transducer.

鱼腥藻感觉视紫红质传感器三种晶型的结晶、X 射线衍射分析和 SIRAS/分子替代定相。

• DOI: 10.1107/s1744309106008359
• 发表时间: 2006
• 期刊: Acta crystallographica. Section F, Structural biology and crystallization communications
• 作者: Vogeley,Lutz;Luecke,Hartmut
• 通讯作者: Luecke,Hartmut

Structural clues to the mechanism of ion pumping in bacteriorhodopsin.

• DOI: 10.1016/s0065-3233(03)63005-6
• 发表时间: 2003
• 期刊: Advances in protein chemistry
• 作者: H. Luecke;J. Lanyi