ISOLATION AND DISTR OF HIGH QUALITY HUMAN PANCRE*

高质量人胰腺的分离和分配*

• 批准号: 6530153
• 负责人: Ali Naji
• 金额: $ 64.01万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6530153
• 关键词: calcium flux; cooperative study; cyclic AMP; diabetes mellitus; diabetes mellitus therapy; glucose metabolism; hormone regulation /control mechanism; human subject; human therapy evaluation; insulin; nonhuman therapy evaluation; pancreatic islet transplantation; pancreatic islets; patient oriented research; postmortem; pulmonary respiration

DESCRIPTION (Provided by applicant): The transplant program at the University of Pennsylvania (UP)has had a long-standing interest in the application of pancreatic islet transplantation in the treatment of diabetes mellitus. The program has continued basic investigations of the biological barriers to islet transplantation, with emphasis on the induction of immunological tolerance. Insights gained from experimental islet transplantation have led to recent successful applications of this therapy and achievement of euglycemia in patients with Type I diabetes mellitus. Based on these advances, a comprehensive islet transplantation program was established at the UP integrating a multidisciplinary team of investigative scientists and clinicians with recognized expertise in diabetes research and transplantation. Since the inception of the program in 1999, significant progress has been made in building its critical components: 1) construction of an Food and Drug Administrative (FDA)-compliant current good manufacturing practices (cGMP) facility devoted exclusively to islet processing; 2) recruitment and training of personnel qualified to perform islet isolation and assignment of dedicated transplant surgeons for on-site recovery of cadaveric pancreata; 3) establishment of a strong collaboration with the local organ procurement organization, which has led to a marked increase in the procurement of the human cadaveric pancreata; 4) distribution of isolated pancreatic islets to regional and national investigators engaged in diabetes research; and 5) development of a standardized islet quality index. In the present application, this strong infrastructure will serve as the mechanism for increasing the availability of human cadaveric pancreata suitable for preparation of isolated islets. This goal will be accomplished by procurement of pancreata from an expanded donor pool, including non-heart-beating organ donors. Another emphasis for the program will be the refinement of the islet quality index, based on physiological, biochemical, and morphological parameters, for correlation with the outcome of islet transplantation. With this strong platform in place, the program is poised to realize its goal of generating large quantities of highly purified and quality-controlled human islets for transplantation into Type I insulin-dependent diabetic patients.

描述（由申请人提供）：大学的移植计划 宾夕法尼亚州（UP）对应用的兴趣长期存在 胰岛移植在糖尿病治疗中。这 计划继续对胰岛的生物壁垒进行基本调查 移植，重点是诱导免疫耐受性。 从实验胰岛移植中获得的见解导致了最近 这种疗法的成功应用和尤利克米亚的实现 I型糖尿病患者。基于这些进步， 在UP建立了全面的胰岛移植计划 整合一个由调查科学家组成的多学科团队 在糖尿病研究和移植方面具有公认的专业知识的临床医生。 自1999年该计划成立以来，已经取得了重大进展 在构建其关键组成部分时：1）建造食物和药物 行政（FDA） - 计划当前良好的制造实践（CGMP） 设施专门用于胰岛处理； 2）招募和培训 有资格执行胰岛隔离和分配专用的人员 移植外科医生，用于现场恢复尸体胰腺； 3） 与当地的器官采购建立强有力的合作 组织，这导致了该组织的显着增加 人尸体胰腺； 4）分离胰岛的分布到 从事糖尿病研究的地区和国家调查人员；和 5）制定标准化胰岛质量指数。 在当前应用中，这种强大的基础设施将作为 增加人尸体胰腺可用性的机制 适合制备孤立的胰岛。这个目标将实现 通过从扩展的供体池中采购胰腺，包括非心跳 器官捐献者。该计划的另一个重点是完善 基于生理，生化和 形态学参数，与胰岛结局相关 移植。有了这个强大的平台，该计划已准备就绪 实现其产生大量高度纯化和的目标 质量控制的人类胰岛将移植到I型胰岛素依赖性 糖尿病患者。