LYSERGIC ACID DIETHYLAMIDE INDUCED GENE EXPRESSION

麦角酸二乙酰胺诱导的基因表达

• 批准号: 6515413
• 负责人: CHARLES D NICHOLS
• 金额: $ 5.01万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6515413
• 关键词: RNase protection assay; antibody formation; gene expression; hallucinogens; in situ hybridization; inhibitor /antagonist; laboratory rabbit; laboratory rat; lysergic acid diethylamide; neuropharmacology; nucleic acid sequence; receptor expression

The specific aims of the research in this proposal are to identify and characterize genes whose expression patterns are influenced by acute LSD administration in the brains of rats. Candidate genes will be identified from a pool of clones isolated in previous Differential Display PCR experiments. Differential expression within the brain for candidates will be determined and characterized by RNase protection and tissue in situ experiments. Antibodies will be generated to analyze candidate protein expression. Differential expression will be tested after administration of different hallucinogens. The receptor mediated pathways in which the differentially expressed genes act will be determined in experiments employing a variety of receptor antagonists. Ultimately these genes, their products and the pathways that are involved may be investigated for their potential role in animal behavior and in human mental disorders such as schizophrenia. This training and research could provide the basis for future work in establishing an independent research program in an academic environment.

该提案中该研究的具体目的是识别和表征其表达模式受大鼠大脑中急性LSD给药影响的基因。将从先前差异显示实验中分离的克隆池中鉴定候选基因。候选者大脑内的差异表达将由RNase保护和原位实验来确定和表征。将生成抗体以分析候选蛋白表达。给药不同的致幻剂后，将测试差异表达。受体介导的途径将在采用多种受体拮抗剂的实验中确定差异表达的基因作用的途径。最终，这些基因，它们的产物和所涉及的途径可能会因其在动物行为和人类精神疾病（例如精神分裂症）中的潜在作用而进行研究。这项培训和研究可以为未来在学术环境中建立独立研究计划的工作提供基础。