Exploratory Data Analysis of Web Quantitative Gel Databa

网络定量凝胶数据库的探索性数据分析

• 批准号: 6559136
• 负责人: peter f lemkin
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6559136
• 关键词: Internet; computer data analysis; computer program /software; computer system design /evaluation; gel electrophoresis; human tissue; information display; information dissemination; proteomics

Early 2D gel data analysis systems required a dedicated stand-alone computer and systems people to install and run the special 2D gel software. We have been interested in extending the access of gel analysis software to a collaborative group setting so that all group members can work on the latest data wherever they are physically located. We have expanded the WebGel system to allow exploratory access of GELLAB-II, GELLAB-II+ (Scanalytics), Melanie-II and other 2D and 1D gel databases to collaborators via the Internet. Users can access their data prepared at the workstation site and copied to their Web server through a Web browser. They can perform various data explorations including spot normalization and statistical comparison of corresponding spots across sets of gels. (For 1D gels, spots would be replaced by bands.) They can compare two gels at a time with Flicker to find significantly changing spots and then look up the corresponding quantitative data with WebGel (http:/www-lecb.ncifcrf.gov/webgel/). WebGel consists of an interdependent set of HTML, CGI-BIN, Java applets, and JavaScript programs (programming languages used to construct Web pages) to access parts of the legacy GELLAB-II quantitative data. The user sees a single Web interface and has transparent access to their own and their collaborators' views of the data. We have been using it to analyze nuclear matrix proteins in prostate cancer gels for tumor markers (Partin, et. al), phosphoproteins (Hornbeck), fetal alcohol syndrome gels (Myrick), and DNA alternations of oncogenes in 2D DNA gels (Patrick). Z01 BC 10259-03

早期的2D凝胶数据分析系统要求专用的独立计算机和系统人员安装和运行特殊的2D凝胶软件。我们一直有兴趣将凝胶分析软件的访问访问到协作组设置，以便所有小组成员可以在物理位置的任何地方处理最新数据。我们已经扩展了WebGel系统，以允许通过Internet通过Internet访问Gellab-II，Gellab-II+（Scanalytics），Melanie-II和其他2D和1D GEL数据库。用户可以访问在工作站网站上准备的数据，并通过Web浏览器复制到其Web服务器。他们可以执行各种数据探索，包括跨凝胶组的相应斑点的斑点归一化和统计比较。 （对于一维凝胶，斑点将被频段替换。）它们可以一次比较两种凝胶与闪烁以查找斑点有显着变化的斑点，然后使用webgel（http：/www-lecb.ncifcrf.gov.gov/webgel/）查找相应的定量数据。 WebGel由一组相互依存的HTML，CGI-BIN，JAVA APPLET和JAVASCRIPT程序（用于构建网页的编程语言）组成，以访问Legacy Gellab-II定量数据的一部分。用户看到一个单个Web界面，并具有透明的访问权限以及其合作者对数据的看法。我们一直在使用它来分析前列腺癌凝胶中的核基质蛋白（Partin等），磷蛋白（Hornbeck），胎儿酒精综合征凝胶（Myrick）和2D DNA凝胶中癌基因的DNA交替。 Z01 BC 10259-03