HOMOCYSTEINE VASCULAR BIOCHEMISTRY & METABOLISM

同型半胱氨酸血管生物化学

• 批准号: 6478947
• 负责人: Donald Weldon Jacobsen
• 金额: $ 5.36万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6478947
• 关键词: AIDS; bacteria; biological products; biomaterials; biomedical resource; carbohydrates; immunology; infection; inflammation; lymphatic system; structural biology; virus

The intercellular matrix (NtkF) of the marine sponge, Microciona prolifera, is a large proteoglycan that is highly glycosylated, sulfated and contains equal amounts of protein and carbohydrate. There is a protein core from which radiate many arms with a high carbohydrate content. Subunits are linked to each other via carbohydrate-carbohydrate bonds, two epitopes of which have been chemically defined. Hyaluronic acid (HA) is a component of MAF, but until now itsfunctional role has been unclear. We have found that MAR as well as MbA.Fds, have potent inhibitory activity against HIV virally infected lymphoid cells and the inhibitory activity I at levels as low as 0. 1 n-&rograms/ml in the absence of any toxicity. We need to sort out, characterize and compare the components of NLAFds in order to identify those with HIV inhibitory effects. The ones which are anionic, i.e., sulfated disaccharide and sulfated tetrasaccharide, may qualify (there maybe others); since these types of anionic compounds have shown HIV inhibition. If their viral inhibitory activity is based upon charge effects, they may prove useful against other enveloped viruses with protein composition similar to HIV. Our recent studies on sponge have shown that sulfation may be an important modifier of cell functions, such as masking of lectins and modulating reactions between proteoglycans and cell surface receptors. The first batch was M[AFds or lyophilized supernatant derived from ethanolic precipitation of M[AFl (water-dialyzed purified M[AF gel that was lyophilized). The second batch was M[AFl (water-dialyzed and lyophilized purified M[AF gel), which was used to derive M[AFds. The mass spectra and GC retention times indicate five monosaccharides with the following relative abundance: Fuc 14%; Man18%; Gal 32%; GlcU 4.5%; GlcNAc; 31%. Neuraminic acid was checked by a very short time methanolysis and none was detected. The total amount of carbohydrate was calculated to be 3.7% - a very small amount.

Microciona海绵的细胞间基质（NTKF） Prolifera是一种大型蛋白聚糖，高度糖基化， 硫化并含有等量的蛋白质和碳水化合物。 有一个蛋白质核心，从中散发出许多臂 碳水化合物含量。 亚基通过 碳水化合物 - 碳水化合物键，两个表位 化学定义。 透明质酸（HA）是MAF的组成部分，但 到目前为止，其功能作用尚不清楚。 我们发现3月 以及MBA.FDS，对HIV具有有效的抑制活性 病毒感染的淋巴样细胞和抑制活性I 在没有任何毒性的情况下，水平低至0。1n-＆rogicals/ml。 我们需要整理，表征和比较NLAFD的组件 为了识别患有艾滋病毒抑制作用的人。 那些 阴离子，即硫酸化二糖和硫酸盐 四糖，可能有资格（也许还有其他）；由于这些类型 阴离子化合物已显示出HIV抑制。 如果他们的病毒 抑制活性基于电荷效应，它们可能证明 针对其他具有蛋白质组成的包膜病毒 类似于艾滋病毒。 我们最近对海绵的研究表明 硫酸化可能是细胞功能的重要修饰符，例如 凝集素的掩盖并调节蛋白聚糖和 细胞表面受体。 第一批是m [afds或冻干 来自M [AFL的乙醇沉淀的上清液 （水 - 二聚纯化的m [被冻干的AF凝胶）。 第二个 批次为m [AFL（水丁二烯和冻干的纯化M [AF凝胶）， 用于推导m [afds。 质谱和GC保留 时间指示五种单糖，以下相对 丰度：FUC 14％；男子18％;加32％; GLCU 4.5％； glcNAC; 31％。 神经氨酸通过很短的甲醇溶解检查，没有 被检测到。 计算的碳水化合物总量为 3.7％ - 少量。