IMMUNOLOGY TESTS

免疫学测试

• 批准号: 6458986
• 负责人: RICHARD A MILLER
• 金额: $ 31.28万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-05 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6458986
• 关键词: T lymphocyte; aging; animal old age; antibody formation; cellular immunity; developmental immunology; erythrocytes; flow cytometry; genetic markers; immunogenetics; immunologic assay /test; laboratory mouse; lymphocyte proliferation; molecular genetics; phenotype

The goal of Project 1 is to assess the immune status of the mice that form the focus of this Program Project, i.e. the 600 UM-HET3 mice in Population 1 and the 180 genotype-selected mice in Population 2. The test battery includes the following components: . Production of anti-erythrocyte antibodies at 4 and again at 15 months of age. . Quantitation of six T cell subsets (CD4 and CD8, CD4 and CD8 memory, and the CD4P and CD8P subsets that express cell surface P-glycoprotein) in blood and in spleen of mice at 18 months of age. . Tests of in vitro proliferation by cultured splenocytes activated by Con A or by anti-CD3 antibodies with or without anti-CD28 co- stimulation. In addition to providing assessments of immune phenotypes for tests of the three Program Aims shared by all of the projects, the data generated by Project 1 will provide answers to several questions about the relationships among T cell subsets, in vitro proliferation, and in vivo immunity in aging mice. These include; 1. Do age-sensitive T cell subset levels in peripheral blood provide a useful index of subset levels in internal lymphoid tissues? 2. Do the factors that lead to age-atypical values of any given T cell subset also regulate the levels of other age-sensitive subsets? For example, do middle-aged mice with high levels of CD4 memory cells, characteristic of advanced age, also exhibit relatively high levels of CD4M, CD4P, CD8P cells? 3. Do mice with relatively "old" levels of T cell subsets show low levels of antibody production and poor responses in tests for in vitro proliferation? In conjunction with other Projects and Cores, these tests for immune status will provide a comprehensive picture of genetic effects on age- sensitive immune traits, and will help test the idea that the pace of immune change in adult life is regulated by factors that also time age- dependent changes in other physiological domains.

项目1的目的是评估构成该程序项目重点的小鼠的免疫状态，即种群1中的600 UM-HET3小鼠和种群中的180种基因型选择的小鼠。测试电池包括以下组件：。 4个月大时，在4和15个月时再次生产抗六抗抗体。 。在18个月大时，对六个T细胞子集（CD4和CD8，CD4和CD8存储器以及表达细胞表面P-糖蛋白的CD4P和CD8P亚群）的定量。 。由CON A或抗CD3抗体激活或不具有抗CD28刺激的抗CD3抗体激活的培养的脾细胞的体外增殖测试。除了对所有项目共享的三个程序的测试评估对免疫表型的评估外，项目1产生的数据还将为有关T细胞子集，体外增殖和体内免疫的几个问题提供答案。这些包括； 1。对外周血中年龄敏感的T细胞子群水平是否提供了内部淋巴组织中亚群水平的有用指数？ 2。导致任何给定T细胞子集的年龄型值的因素是否还调节其他年龄敏感子集的水平？例如，具有高水平CD4记忆细胞的中年小鼠（高级年龄的特征）是否表现出相对较高的CD4M，CD4P，CD8P细胞？ 3。具有相对“旧” T细胞亚群水平的小鼠在体外增殖的测试中的抗体产生水平较低，反应不佳？与其他项目和核心结合使用，这些对免疫状态的测试将提供对年龄敏感免疫特征的遗传影响的全面图景，并将有助于测试成人生活中免疫变化的速度，即成人生活的速度受到其他生理领域的时间年龄变化的因素的调节。