REGULATION OF ENERGY METABOLISM IN MOSQUITOES

蚊子能量代谢的调节

• 批准号: 6497303
• 负责人: MICHAEL A WELLS
• 金额: $ 31.88万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-15 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6497303
• 关键词: Aedes; Anopheles; aminoacid metabolism; bioenergetics; carbon dioxide; dietary aminoacid; enzyme activity; female; glycogen; glycogenolysis; lipids; nutrition; nutrition related tag; oogenesis; phosphoenolpyruvate carboxylase; protein metabolism

DESCRIPTION: Female mosquitoes require a blood meal from a vertebrate host to produce eggs. The public health importance of mosquitoes derives from the fact that while obtaining such a blood meal from a human host the mosquito is able to transmit diseases, such as malaria and dengue, which world-wide are responsible for millions of deaths per year. We need to significantly increase our understanding of the biology of mosquitoes. One area that has been neglected, so far, is fundamental metabolic studies directed towards understanding how the female mosquito utilizes the components of the blood meal. At most 20 percent of the blood meal amino acids are used for egg production and the purpose of this proposal is determine the fate of the other 80 percent. Thus, this proposal is concerned with following the fate amino acids derived from blood meal proteins under a variety of nutritional states. In addition, we propose to assess the role of preexisting energy reserves in the female mosquito during blood meal utilization. Once the relevant pathways have been defined, future studies will be concerned with mechanisms that regulate the pathways under different nutritional, behavioral and environmental conditions. In this proposal we plan to quantitatively examine the fate of amino acids derived from blood meal proteins in Aedes aegypti, the vector of dengue fever and Anopheles albimanus, a vector of malaria in Central and South America. The following specific aims are proposed: 1. Determine the metabolic fate of 14C-labeled blood meal protein amino acids. 2. Determine the metabolic fate of preexisting energy stores, specifically glycogen and lipid, following a blood meal. 3. Characterize the metabolism of blood meal protein amino acids.

描述：雌性蚊子需要脊椎动物宿主的血餐 产生鸡蛋。蚊子的公共卫生重要性源于事实 在从人类宿主那里获得这样的血餐时，蚊子能够 传播疾病，例如疟疾和登革热，全球 每年造成数百万次死亡。我们需要显着增加 我们对蚊子生物学的理解。一个地方 到目前为止，被忽视的是针对的基本代谢研究 了解女性蚊子如何利用血液的成分 一顿饭。最多20％的血液粉氨基酸用于鸡蛋 生产和该提议的目的是确定对方的命运 80％。因此，该提议与遵循命运氨基有关 从多种营养态下衍生出血液粉蛋白的酸。 此外，我们建议评估已经存在的能源储备在 血液粉利用过程中的雌性蚊子。一旦相关途径 已经定义了，未来的研究将关注 调节不同营养，行为和环境的途径 状况。在此提案中，我们计划定量检查 源自伊德斯伊德斯（Aedes aegypti）的血液粉蛋白的氨基酸， 登革热和阿尔皮利斯（Albimanus），中央和南部的疟疾载体 美国。 提出了以下特定目的：1。确定代谢命运 14C标记的血液粉蛋白氨基酸。 2。确定代谢命运 先前存在的能量存储，特别是糖原和脂质，后血液 一顿饭。 3。表征血粉蛋白氨基酸的代谢。