CENTER FOR STRUCTURAL GENOMICS

结构基因组学中心

• 批准号: 6503363
• 负责人: JEFFREY B BONANNO
• 金额: $ 31万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6503363
• 关键词: Escherichia coli; Internet; Saccharomyces cerevisiae; X ray crystallography; bacterial proteins; biomedical automation; biotechnology; computer program /software; computer system design /evaluation; cooperative study; data collection; data collection methodology /evaluation; functional /structural genomics; genetic library; high performance liquid chromatography; high throughput technology; information dissemination; mass spectrometry; model design /development; molecular biology information system; molecular cloning; protein purification; protein structure function; technology /technique development; ultraviolet spectrometry

DESCRIPTION (Taken from the Application): The Albert Einstein College of Medicine, the Department of Biology at Brookhaven National Laboratory, Mount Sinai School of Medicine, the Rockefeller University, and the Weill Medical College of Cornell University have formed the New York Structural Genomics Research Consortium (NYSGRC) for high-throughput, structural and functional studies of proteins. The research program takes the form of a Pilot Study focused on establishing a fully-integrated, high-throughput system for protein family classification and target selection, target protein expression, purification, biophysical characterization and crystallization, target protein structure determination by X-ray crystallography, and analyses and dissemination of the results. The long-term goal of the NYSGRC is to create an efficient, cost-effective Structural Genomics Center that will contribute substantially to the task of determining the 10,000 plus three-dimensional protein structures envisaged by the National Institute of General Medical Sciences Protein Structure Initiative. The specific aims of the NYSGRC application are: Aim 1. Identify target protein sequences for structural genomics. Aim 2. Develop high-throughput E. coli expression of soluble target proteins. Aim 3. Develop high-throughput production of target proteins. Aim 4. Develop high-throughput biophysical characterization of target proteins. Aim 5. Develop high-throughput crystallization of target proteins. Aim 6. Develop efficient experimental strategies for MAD crystallography. Aim 7. Develop high-throughput synchrotron data collection with target protein crystals. Aim 8. Develop and use an Internet-based computational pipeline for protein crystallography. Aim 9. Develop high-throughput molecular replacement tools for crystallography. Aim 10 Develop high-throughput comparative modeling for structural genomics. Aim 11. Develop efficient annotation and dissemination of protein structures and models. Aim 12. Develop an Internet-based "web-book" for use by the NYSGRC.

描述（摘自申请表）：阿尔伯特·爱因斯坦学院 医学，布鲁克海文国家实验室生物学系，蒙特 西奈医学院、洛克菲勒大学和威尔医学院 康奈尔大学学院成立了纽约结构基因组学研究所 高通量、结构和功能研究联盟 (NYSGRC) 蛋白质的研究。该研究计划采取试点研究的形式 专注于建立一个完全集成的、高通量的蛋白质系统 家族分类和靶标选择、靶蛋白表达、 纯化、生物物理表征和结晶、目标蛋白 通过 X 射线晶体学确定结构，并进行分析和 传播结果。 NYSGRC 的长期目标是创建一个 高效、具有成本效益的结构基因组学中心将做出贡献 实质上是为了确定 10,000 多个三维空间的任务 美国国家普通医学研究所设想的蛋白质结构 科学蛋白质结构倡议。 NYSGRC 申请的具体目标是： 目标 1. 鉴定结构基因组学的靶蛋白序列。 目标 2. 开发可溶性靶蛋白的高通量大肠杆菌表达。 目标 3. 开发目标蛋白的高通量生产。 目标 4. 开发目标蛋白的高通量生物物理表征。 目标 5. 开发目标蛋白的高通量结晶。 目标 6. 开发有效的 MAD 晶体学实验策略。 目标 7. 开发目标蛋白的高通量同步加速器数据收集 晶体。 目标 8. 开发并使用基于互联网的蛋白质计算管道 晶体学。 目标 9. 开发用于晶体学的高通量分子替代工具。 目标 10 开发结构基因组学的高通量比较模型。 目标 11. 开发蛋白质结构的有效注释和传播 和模型。 目标 12. 开发一本基于互联网的“网络书”，供 NYSGRC 使用。