CORE--TISSUE PROCESSING FACILITY

核心——组织处理设施

• 批准号: 6487290
• 负责人: ANTHONY A GASPARI
• 金额: $ 15万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6487290
• 关键词: biomedical facility; clinical research; human papillomavirus; human subject; tissue resource /registry; viral vaccines; warts

The Tissue Processing Core will be responsible for the coordination of all aspects of dispersing human tissues for the proposed program project. The Tissue Processing Core will be composed of the Core Director, Anthony A. Gaspari, MD; Robert Rose, PhD, Glynis Scoot, MD, Consultant, and a technical associate, Ms. Carol Tanck. The Specific Aims of the core will be to: 1) Recruit subjects with external genital warts to obtain wart tissue 2) Recovery and expansion of wart-infiltrating lymphocytes for in vitro immunologic studies. 3) Processing and storage of peripheral blood lymphocytes. 4) Processing and storage of serum samples. 5) HPV Genotyping by DNA PCR-ELISA 6) Preparation of autologous HPV vaccine from HPV infected tissue. 7) Coordinate distribution of tissues to appropriate investigators.

组织处理核心将负责协调拟议项目中人体组织分散的各个方面。组织处理核心将由核心主任 Anthony A. Gaspari（医学博士）组成； Robert Rose 博士、Glynis Scoot 医学博士、顾问和技术助理 Carol Tanck 女士。该核心的具体目标是： 1) 招募患有外生殖器疣的受试者以获得疣组织 2) 恢复和扩增疣浸润淋巴细胞以用于体外免疫学研究。 3）外周血淋巴细胞的处理和保存。 4) 血清样本的处理和保存。 5) 通过 DNA PCR-ELISA 进行 HPV 基因分型 6) 从 HPV 感染组织中制备自体 HPV 疫苗。 7) 协调组织向适当的研究者的分配。