CORE--MOLECULAR BIOLOGY FACILITY

核心--分子生物学设施

• 批准号: 6459551
• 负责人: ANDREW Abba STOLZ
• 金额: $ 21.25万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6459551
• 关键词: Xenopus oocyte; biomedical facility; complementary DNA; expression cloning; genetic transcription; molecular biology; molecular biology information system; nucleic acid sequence; polymerase chain reaction; recombinant proteins; transfection /expression vector

DESCRIPTION (Taken from the application) The Molecular Biology Core facility is composed of three components plus a sub-core directed by Dr. Kasahara, who will supervise generation of viral based gene delivery systems. The Molecular Biology Core facility supports three major classes of activities. The first major class of activity is focused on developing specific reagents to express cDNAs of interest. We are a campus wide resource for preparation of Xenopus laevis oocytes for RNA based expression studies and bacterial expression of recombinant proteins. We have added baculovirus expression and are developing inducible regulated expression systems for mammalian cell lines. In addition, we have planned a new sub-core directed by the new Co- Director, who will direct development of viral based gene delivery systems. This component also performs all the subcloning activities required to support the different expression systems listed above. The second component is a Molecular Biology Guidance and Resource Center, which introduces and guides investigators in utilizing molecular biological techniques who have no or limited experience. Included in these services are searching databases for sequence homologies and designing primers for PCR amplification and DNA sequencing. The third component of the Molecular Biology Core facility is operational management of sophisticated equipment needed in routine molecular biological studies.

描述（取自应用程序）分子生物学核心设施由三个组件以及由卡萨哈拉博士指导的子核心组成，后者将监督基于病毒的基因输送系统的产生。分子生物学核心设施支持三个主要的活动。第一类主要活动的重点是开发特定的试剂以表达感兴趣的cDNA。我们是为基于RNA的表达研究和重组蛋白的细菌表达制备制备Xenopus laevis卵母细胞的校园广泛资源。我们已经添加了杆状病毒表达，并正在开发可诱导的哺乳动物细胞系调节表达系统。此外，我们还计划了由新的合作社指导的新子核心，该核心将指导基于病毒的基因输送系统的开发。该组件还执行支持上面列出的不同表达系统所需的所有子克隆活动。第二部分是分子生物学指导和资源中心，该中心介绍并指导了研究人员利用没有经验或有限经验的分子生物学技术。这些服务中包含的是搜索数据库中的序列同源性和设计用于PCR扩增和DNA测序的引物。分子生物学核心设施的第三个组成部分是对常规分子生物学研究所需的复杂设备的运营管理。