Enhanced Local Lymph Node Assay Using Flow Cytometry

使用流式细胞术增强局部淋巴结检测

• 批准号: 6484384
• 负责人: George L. DeGeorge
• 金额: $ 31.34万
• 依托单位: MB RESEARCH LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2004-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6484384
• 关键词: alternatives to animals in research; biomarker; bromodeoxyuridine; contact dermatitis; flow cytometry; immunologic assay /test; immunotoxicity; laboratory mouse; lymph nodes; lymphocyte proliferation; technology /technique development

DESCRIPTION (Applicant?s abstract): Although the murine Local Lymph Node Assay (LLNA) is effective at detecting potential irritants/sensitizers, this assay, in its current standard form: 1) cannot readily differentiate some types of irritants from respiratory and contact sensitizers; 2) utilizes moderate amounts of radioactive material and; 3) requires increased numbers of animals to determine the different endpoints (i.e., proliferation, immunophenotype) to fully characterize the response of an animal to a topically-applied chemical. To enhance and improve this important test, our company has implemented several innovative modifications to the standard LLNA. We have applied flow cytometric techniques to this assay to increase the sensitivity/specificity of the assay, eliminate the use of radioactive material and decrease the number of animals used for screening. It has been determined by the results of SBIR Phase I studies that the proposed enhanced flow cytometry-based LLNA is technically and commercially feasible. However, to develop this assay to its full commercial potential, which our company would provide to the chemical, pharmaceutical and consumer products industries, our group must: 1) validate the enhanced version of the LLNA; 2) establish the cytometry-based LLNA as a GLP compliant assay at our company and; 3) develop and provide various versions of the enhanced LLNA for our clients.

描述（申请人的摘要）：尽管鼠本地淋巴结测定法 （LLNA）有效地检测潜在的刺激性/敏化剂，该测定法 以当前标准形式：1）无法轻易区分某些类型的 来自呼吸和接触敏化剂的刺激性； 2）利用中等 放射性材料量和； 3）需要增加动物数量 确定不同的终点（即增殖，免疫表型）至 充分表征动物对局部应用化学物质的反应。 为了增强和改进这一重要测试，我们公司已实施了几项 对标准LLNA的创新修改。我们已经应用流式细胞术 该测定的技术提高了测定的灵敏度/特异性， 消除放射性材料的使用并减少动物的数量 用于筛选。它已由SBIR I期的结果确定 研究表明，提出的增强基于流式细胞仪的LLNA在技术上是 商业上可行。但是，将此测定开发为完整的广告 潜力，我们公司将向化学，药物和 消费产品行业，我们的小组必须：1）验证增强版本 llna; 2）建立基于细胞术的LLNA作为符合GLP的测定法 我们的公司3）开发和提供各种增强的LLNA 为我们的客户。