CTL responses to vaccination in CRPV rabbit model

CRPV 兔模型中 CTL 对疫苗接种的反应

• 批准号: 6445484
• 负责人: JANET L BRANDSMA
• 金额: $ 7.23万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6445484
• 关键词: cell mediated lymphocytolysis test; cell transformation; cytotoxic T lymphocyte; disease /disorder model; laboratory rabbit; neoplasm /cancer vaccine; rabbit papillomavirus; technology /technique development; viral vaccines

Cervical cancer is caused by infection with high-risk human papillomaviruses (HPVs). The viral etiology of this cancer indicates that it should be possible to develop highly specific and effective antiviral and antitumor vaccines. The control of HPV infection and associated lesions is believer to be mediated through the action of cytotoxic T cells (CTLs). The only small animal model for the study of high risk (cancer-associated) papillomavirus infection is the cottontail rabbit papillomavirus (CRPV)-rabbit model. Using this model, my laboratory has produced a number of CRPV vaccines that protect rabbits against primary CRPV infection or that suppress the clinical manifestations of a preestablished infection. Our ability to advance beyond the current in vivo results is hindered by the fact that a CTL assay for rabbits is currently not available. Since recent advances in immunology make it feasible to develop a rabbit CTL assay, this is the goal of our project. Our specific aims are: 1) to produce a source of immortal cells for the development of CTL targets; 2) to generate antigen-specific target cells; 3) to develop a CTL assay using effector cells from vaccinated rabbits.

宫颈癌是由高危人乳头瘤病毒（HPV）感染引起的。该癌症的病毒病因表明，应该有可能开发高度特异性和有效的抗病毒和抗肿瘤疫苗。 HPV感染和相关病变的控制是通过细胞毒性T细胞（CTL）的作用来介导的。研究高风险（与癌症相关的）乳头瘤病毒感染的唯一小动物模型是棉尾兔乳头瘤病毒（CRPV） - 兔模型。使用此模型，我的实验室生产了许多CRPV疫苗，可保护兔子免受原发性CRPV感染或抑制预先建立的感染的临床表现。 目前尚无兔子的CTL测定法，我们无法超越当前体内效果的能力。 自从免疫学的最新进展使得开发兔子CTL测定法可能是可行的，这是我们项目的目标。我们的具体目的是：1）产生一种不朽的细胞来源，以开发CTL目标； 2）生成抗原特异性靶细胞； 3）使用来自接种兔子的效应细胞开发CTL分析。