FUNCTIONL ANALYSIS OF M1 METALLO AMINOPEPTIDASE GENE FAMILY IN YEAST

酵母M1金属氨肽酶基因家族的功能分析

• 批准号: 6442290
• 负责人: Daniel Caprioglio
• 金额: $ 28.07万
• 依托单位: COLORADO STATE UNIVERSITY-PUEBLO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6442290
• 关键词: Saccharomyces cerevisiae; affinity chromatography; aminopeptidase; cell biology; cell growth regulation; enzyme activity; fluorescence microscopy; fungal genetics; fusion gene; gene interaction; gene mutation; genetic regulation; green fluorescent proteins; ion exchange chromatography; metalloenzyme; molecular cloning; nucleic acid sequence; physiologic stressor; polymerase chain reaction; protein purification; protein structure function

This project proposes to investigate the role of the M1 metallo- aminopeptidase gene family in the cellular physiology of the yeast Saccharomyces cerevisiae. Although aminopeptidase genes or gene products are thought to be involved in a number of disease states (small lung cell carcinomas, renal carcinomas, pre-B cell lymphomas, and others), not much is understood about their role in cellular physiology. Using the power of yeast genetics, strains will be developed containing one or more M1 metallo-aminopeptidase genes disrupted. The strains will be analyzed for defects ingrowth, physiology, and response to stress. The project will use genefusion and fluorescent microscopy techniques to localized the gene products in the cell. The project will also analyze two of the metallo-aminopeptidase gene products that have not been isolated or characterized in their activity and specificity. Finally, the project will isolate suppressors of the phenotypic lesions casued by metallo-aminopeptidase gene disruptions. This will allow analysis of how the metallo-aminopeptidase cause the changes in the cell. The elucidation of aminopeptidase function is important in understanding how aminopeptidases may be involved in the diseases above.

该项目建议研究M1金属的作用 酵母细胞生理学中的氨基肽酶基因家族 酿酒酵母。 虽然氨基肽酶基因或基因 人们认为产品参与了许多疾病状态 （小肺细胞癌，肾癌，前B细胞淋巴瘤， 和其他），关于它们在细胞中的作用并不多 生理。 使用酵母遗传学的力量，菌株将是 开发了一个或多个M1金属氨基肽酶基因 破坏了。 菌株将被分析以使缺陷向内生长， 生理学和对压力的反应。 该项目将使用GeneFusion 和荧光显微镜技术局部化基因产物 在细胞中。 该项目还将分析两个金属氨基肽酶基因 尚未隔离或表征的产品 活动和特异性。 最后，该项目将隔离表型的抑制剂 金属 - 氨基肽酶基因破坏的病变。 这会 允许分析金属氨基肽酶如何引起变化 细胞。 阐明氨基肽酶功能在 了解氨基肽酶如何参与疾病 多于。