SENSORY ORGAN FORMATION/ GENE EXPRESSION --INNER EAR DEVELOPMENT

感觉器官形成/基因表达——内耳发育

• 批准号: 6469249
• 负责人: ELBA E SERRANO
• 金额: $ 6.87万
• 依托单位: NEW MEXICO STATE UNIVERSITY LAS CRUCES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-01 至 2002-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6469249
• 关键词: Xenopus; auditory pathways; confocal scanning microscopy; developmental neurobiology; ear hair cell; electron microscopy; ganglion cell; gene expression; genetic library; histogenesis; in situ hybridization; molecular cloning; tissue /cell culture; vestibular apparatus; voltage /patch clamp

The goal of this research is to elucidate the cellular and molecular processes that underlie sensory organ formation during inner ear development. The proposed research will use the auditory and vestibular system of Xenopus laevis and Xenopus (Silurana) tropicalis as experimental models. Results are expected to provide comparative data about the structure, growth, and genetic profile of three inner ear endorgans: the sacculus (an acoustico-vestibular organ) and the amphibian papilla (an acoustic organ). Experiments will focus on cells of the peripheral nervous system: mechanosensory hair cells and sensory ganglion cells. A major long term goal of this research is to develop technologies and methods for identifying novel genes expressed in the inner ear. The specific aims are: (1) to obtain methods for identifying novel genes expressed in the inner ear. The specific aims are: (1) to obtain comparative data about pattern formation during development of the sensory epithelial of the utricle, amphibian papilla, and sacculus, (2) to identify the sites of hair cell and sensory ganglion cell production during ear development, (3) to synthesize cDNA libraries from the inner ear of X. laevis and X. tropicalis, and (4) to analyze gene expression patterns of inner ear cDNA library clones. Methods used in this research include RT-PCR directed cDNA library construction, isolation of RNA from small quantities of inner ear tissue, in situ hybridization, bioinformatics, and multi-photon laser scanning confocal microscopy. Experimental results will provide fundamental of data about the pattern of hair cell differentiation and proliferation during sensory organ formation and will advance understanding of gene expression during inner ear development. The long term goal of this research is to identify the function of novel genes expressed in the inner ear, and to determine how genes expressed in the inner ear are regulated during development, regeneration, and aging. Results of these investigations will provide essential knowledge that can be used to develop effective treatments for hereditary and environmentally-induced disorders of hearing and balance.

这项研究的目的是阐明内耳发育过程中感觉器官形成的细胞和分子过程。拟议的研究将使用非洲爪蟾和热带爪蟾的听觉和前庭系统作为实验模型。预计结果将提供有关三个内耳内器官的结构、生长和遗传特征的比较数据：球囊（一种声学前庭器官）和两栖动物乳头（一种声学器官）。实验将集中于周围神经系统的细胞：机械感觉毛细胞和感觉神经节细胞。这项研究的一个主要长期目标是开发识别内耳表达的新基因的技术和方法。具体目标是：（1）获得鉴定内耳表达的新基因的方法。具体目标是：（1）获得椭圆囊、两栖动物乳头和球囊感觉上皮发育过程中模式形成的比较数据，（2）确定耳朵发育过程中毛细胞和感觉神经节细胞产生的部位， (3)合成来自非洲小蠹和热带小蠹内耳的cDNA文库，以及(4)分析内耳cDNA文库克隆的基因表达模式。本研究中使用的方法包括 RT-PCR 指导的 cDNA 文库构建、从少量内耳组织中分离 RNA、原位杂交、生物信息学和多光子激光扫描共聚焦显微镜。实验结果将提供有关感觉器官形成过程中毛细胞分化和增殖模式的基础数据，并将促进对内耳发育过程中基因表达的理解。这项研究的长期目标是确定内耳中表达的新基因的功能，并确定内耳中表达的基因在发育、再生和衰老过程中如何受到调节。这些调查的结果将提供必要的知识，可用于开发针对遗传性和环境引起的听力和平衡障碍的有效治疗方法。