CARIBBEAN OCTOCORAL--SOURCE FOR ANTITUBERCULAR COMPOUNDS

加勒比八珊瑚——抗结核化合物的来源

• 批准号: 6472788
• 负责人: ABIMAEL D RODRIGUEZ
• 金额: $ 11.03万
• 依托单位: UNIVERSITY OF PUERTO RICO RIO PIEDRAS
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6472788
• 关键词: Caribbean islands; X ray crystallography; animal extract; antitubercular agents; aquatic organism; biological products; chemical structure function; coral; drug discovery /isolation; drug screening /evaluation; field study; gas chromatography; infrared spectrometry; laboratory mouse; liquid chromatography; mass spectrometry; nuclear magnetic resonance spectroscopy; saltwater environment; ultraviolet spectrometry

Description (Adapted from Application): Marine invertebrates have been identified for a long time by chemists as likely sources of new biomedicinal substances. Pharmacologists, physiologists, and biochemists have demonstrated that many of these novel marine products modify fundamental life processes in ways suggesting biomedical applications. These molecules can serve as leads to guide the pharmaceutical and chemical industries in developing new products. This research aims specifically at Caribbean gorgonian octocorals as a likely resource for novel anti-tuberculosis natural products. Since less than 20 percent of the species of gorgonian octocorals known to exist in the Caribbean region have been assessed chemically for their secondary metabolite composition, and many of these have been shown to produce metabolites possessing potent chemotherapeutic properties, gorgonians from this region have the potential to contain a wealth of novel drugs. Specifically, during the four years of this research, the investigators plan to carefully scrutinize the pharmacology and chemistry of 40 or so representative species belonging to all the major Caribbean gorgonian genera. Initial extraction with solvents of different polarities (e.g., hexane, methylene chloride, and methanol) will be monitored using anti-tuberculosis activity assays. Only gorgonian extract residues possessing strong anti-tuberculosis properties will be screened further for chemical structural features by 1H- and 13C-NMR spectroscopy. Further purification by chromatography (column, GC, LC) and determination of molecular structure will proceed if warranted by the screening results. Structure elucidation will rely heavily on spectral methods (e.g., 1H- and 13C-NMR, MS, IR, UV) and X-ray diffraction techniques.

描述（根据应用程序改编）：海洋无脊椎动物已经 化学家很长时间以新生物医学来源确定了很长时间 物质。药理学家，生理学家和生物化学家已经证明 这些新颖的海洋产品中有许多修改了基本的生活过程 建议生物医学应用的方法。这些分子可以用作 指导制药和化学工业开发新产品。 这项研究专门针对加勒比海gorgonian八角形 新型抗结核天然产物的资源。由于不到20 已知存在于加勒比海已知的八角形物种的百分比 该区域已被化学评估其继发代谢物 组成，其中许多已显示出产生代谢物 拥有有效的化学治疗特性，来自该地区的Gorgonians具有 包含大量新型药物的潜力。具体而言，在四个期间 多年的研究，研究人员计划仔细审查 所有属于所有人的40种代表性物种的药理学和化学 加勒比海的主要戈尔贡属。最初提取的溶剂 不同的极性（例如己烷，甲基氯化物和甲醇）将是 使用抗结核活性测定法监测。仅Gorgonian提取物 将筛选具有强抗结核特性的残留物 1H和13C-NMR光谱的化学结构特征进一步。 通过色谱（柱，GC，LC）进一步纯化，并确定 如果通过筛选结果保证，将进行分子结构。 结构阐明将在很大程度上依赖光谱方法（例如1H-和 13C-NMR，MS，IR，UV）和X射线衍射技术。