EXTRACELLULAR MATRIX AND AXONAL GUIDANCE IN C. ELEGANS

线虫的细胞外基质和轴突引导

• 批准号: 6539795
• 负责人: WILLIAM G WADSWORTH
• 金额: $ 31.12万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-05-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6539795
• 关键词: Caenorhabditis elegans; chimeric proteins; complementary DNA; developmental neurobiology; entactin; extracellular matrix proteins; gene mutation; genetic promoter element; immunocytochemistry; neuroanatomy; neuronal guidance; nucleic acid sequence; site directed mutagenesis

DESCRIPTION (Verbatim From the Applicant's Abstract): Only recently have molecules that guide axon migration been discovered. One of the first was the UNC-6 netrin from the nematode Caenorhabditis elegans. Other guidance molecules interact with and affect UNC-6 activity, and their discovery and the genetic dissection of their interactions are a main focus of this application. Examples include a C. elegans nidogen that acts with UNC-6 to establish the axon scaffold and the identification of a domain within UNC-6 (the C domain) that appears to inhibit axonal branching (branching occurs when this domain is deleted in an UNC-6deltaC construct). The goals of this application are 1) to determine activities associated with other structural modules in UNC-6; 2) to characterize suppressors of UNC-6deltaC; 3) to characterize two other genes that were identified in the screen that identified the C. elegans nidogen; and 4) to identify genes that are required for nidogen to position longitudinal nerves.

描述（逐字研究申请人的摘要）：直到最近才有 发现指导轴突迁移的分子。第一个是 线虫秀丽隐杆线虫的UNC-6 Netrin。其他指导分子 与UNC-6活性及其发现及其发现和遗传 解剖其相互作用是该应用程序的主要重点。例子 包括秀丽隐杆线虫的nidogen，该nidogen与UNC-6作用以建立轴突 脚手架和在UNC-6（C域）内识别一个域 似乎抑制轴突分支（当该域为时发生分支 在UNC-6Deltac构建体中删除）。该应用程序的目标是1） 确定与UNC-6中其他结构模块相关的活动； 2）到 特征是UNC-6DELTAC的抑制器； 3）表征另外两个基因 在鉴定出秀丽隐杆线虫nidogen的屏幕上被鉴定出来；和 4）识别尼多基所需的基因以定位纵向 神经。