CI-2 Modulation of Spinal Processes: Supraspinal Effects

CI-2 脊柱过程的调节：椎上效应

• 批准号: 6541767
• 负责人: ROBERT D FOREMAN
• 金额: $ 27.84万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6541767
• 关键词: amygdala; electromyography; electrophysiology; fos protein; histology; ibotenate; immunocytochemistry; interneurons; laboratory rat; lumbosacral region; membrane potentials; neural information processing; neuroanatomy; neurophysiology; neuroregulation; parabrachial nucleus; respiration regulatory center; sensorimotor system; spinal cord; vagus nerve; visceral afferent nerve

DESCRIPTION (provided by applicant): The purpose of this study is to examine how neurons of propriospinal pathway(s) originating in the C1-C2 region process information from amygdala, subcoeruleus/parabrachial (SC/PB) nuclei, and vagal afferent fibers to modulate sensory-motor integration in the spinal cord. We previously demonstrated that chemical stimulation of C1-C2 neurons modulated spontaneous and visceral-evoked activity in lumbosacral spinal neurons and EMG activity of thoracic paraspinal muscles. Our preliminary data further demonstrate that chemical stimulation of C1 C2 neurons can strongly influence the activity of T3-T4 respiratory-related interneurons. Especially critical to this application are our preliminary results indicating that excitotoxic blockade of C1-C2 neurons with ibotenic acid, attenuated amygdalar and SC/PB modulation of lumbosacral spinal cells. The same lesion reduced vagal effects on lumbosacral neurons and paraspinal muscles. Our results challenge the assumption that descending pathways from supraspinal regions modulate activity of thoracic and lumbosacral neurons through direct projections only. The present application addresses the hypothesis that C1-C2 neurons process information from amygdala, SC/PB and vagal afferents. In turn, C1-C2 neurons strongly influence activity of spinal sensory neurons, thoracic respiration-related interneurons, and somatomotor reflexes. We also hypothesize that amygdala and vagal afferents transmit information to C1-C2 neurons via SC/PB nuclei. Specific aims are designed to answer the following questions: 1) Are discharge patterns and activities of C1-C2 neurons affected by stimulating specific supraspinal nuclei? 2) Do neurons in C1-C2 segments process information from specific supraspinal nuclei to change sensory and integrative/motor activity in the spinal cord? 3) Do neurons in C1-C2 segments process information from vagal afferents to change integrative/motor and sensory activity in the spinal cord? 4) Do SC/PB nuclei relay information from amygdala and vagal afferent fibers to the C1-C2 segments to change sensory and integrative/motor activity in the spinal cord? Neurophysiological studies to examine extracellular discharge patterns and studies using c-fos as a marker of neuronal activation will be conducted in anesthetized rats.

描述（由申请人提供）：本研究的目的是检查起源于 C1-C2 区域的本体脊髓通路的神经元如何处理来自杏仁核、蓝核下/臂旁 (SC/PB) 核和迷走神经传入纤维的信息，从而调节脊髓中的感觉运动整合。我们之前证明，C1-C2 神经元的化学刺激可调节腰骶脊髓神经元的自发和内脏诱发活动以及胸椎旁肌的肌电图活动。我们的初步数据进一步表明，C1 C2 神经元的化学刺激可以强烈影响 T3-T4 呼吸相关中间神经元的活动。对于该应用特别重要的是我们的初步结果表明，鹅膏蕈酸对 C1-C2 神经元有兴奋性毒性阻断，减弱了杏仁核和腰骶脊髓细胞的 SC/PB 调节。相同的病变减少了迷走神经对腰骶神经元和椎旁肌的影响。我们的结果挑战了这样的假设，即来自脊髓上区域的下行通路仅通过直接投射来调节胸椎和腰骶神经元的活动。本申请提出了以下假设：C1-C2神经元处理来自杏仁核、SC/PB和迷走神经传入的信息。反过来，C1-C2 神经元强烈影响脊髓感觉神经元、胸部呼吸相关中间神经元和躯体运动反射的活动。我们还假设杏仁核和迷走神经传入通过 SC/PB 核向 C1-C2 神经元传递信息。具体目标旨在回答以下问题：1）刺激特定脊髓上核会影响C1-C2神经元的放电模式和活动吗？ 2) C1-C2 节段的神经元是否处理来自特定脊髓上核的信息以改变脊髓的感觉和整合/运动活动？ 3) C1-C2 节段中的神经元是否处理来自迷走神经传入的信息以改变脊髓中的整合/运动和感觉活动？ 4) SC/PB 核是否将信息从杏仁核和迷走神经传入纤维传递到 C1-C2 节段，以改变脊髓的感觉和整合/运动活动？将在麻醉大鼠中进行检查细胞外放电模式的神经生理学研究以及使用 c-fos 作为神经元激活标记的研究。