CI-2 Modulation of Spinal Processes: Supraspinal Effects

CI-2 脊柱过程的调节：椎上效应

• 批准号: 6541767
• 负责人: ROBERT D FOREMAN
• 金额: $ 27.84万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6541767
• 关键词: amygdala; electromyography; electrophysiology; fos protein; histology; ibotenate; immunocytochemistry; interneurons; laboratory rat; lumbosacral region; membrane potentials; neural information processing; neuroanatomy; neurophysiology; neuroregulation; parabrachial nucleus; respiration regulatory center; sensorimotor system; spinal cord; vagus nerve; visceral afferent nerve; amygdala; electromyography; electrophysiology; fos protein; histology; ibotenate; immunocytochemistry; interneurons; laboratory rat; lumbosacral region; membrane potentials; neural information processing; neuroanatomy; neurophysiology; neuroregulation; parabrachial nucleus; respiration regulatory center; sensorimotor system; spinal cord; vagus nerve; visceral afferent nerve

DESCRIPTION (provided by applicant): The purpose of this study is to examine how neurons of propriospinal pathway(s) originating in the C1-C2 region process information from amygdala, subcoeruleus/parabrachial (SC/PB) nuclei, and vagal afferent fibers to modulate sensory-motor integration in the spinal cord. We previously demonstrated that chemical stimulation of C1-C2 neurons modulated spontaneous and visceral-evoked activity in lumbosacral spinal neurons and EMG activity of thoracic paraspinal muscles. Our preliminary data further demonstrate that chemical stimulation of C1 C2 neurons can strongly influence the activity of T3-T4 respiratory-related interneurons. Especially critical to this application are our preliminary results indicating that excitotoxic blockade of C1-C2 neurons with ibotenic acid, attenuated amygdalar and SC/PB modulation of lumbosacral spinal cells. The same lesion reduced vagal effects on lumbosacral neurons and paraspinal muscles. Our results challenge the assumption that descending pathways from supraspinal regions modulate activity of thoracic and lumbosacral neurons through direct projections only. The present application addresses the hypothesis that C1-C2 neurons process information from amygdala, SC/PB and vagal afferents. In turn, C1-C2 neurons strongly influence activity of spinal sensory neurons, thoracic respiration-related interneurons, and somatomotor reflexes. We also hypothesize that amygdala and vagal afferents transmit information to C1-C2 neurons via SC/PB nuclei. Specific aims are designed to answer the following questions: 1) Are discharge patterns and activities of C1-C2 neurons affected by stimulating specific supraspinal nuclei? 2) Do neurons in C1-C2 segments process information from specific supraspinal nuclei to change sensory and integrative/motor activity in the spinal cord? 3) Do neurons in C1-C2 segments process information from vagal afferents to change integrative/motor and sensory activity in the spinal cord? 4) Do SC/PB nuclei relay information from amygdala and vagal afferent fibers to the C1-C2 segments to change sensory and integrative/motor activity in the spinal cord? Neurophysiological studies to examine extracellular discharge patterns and studies using c-fos as a marker of neuronal activation will be conducted in anesthetized rats.

描述（由申请人提供）：这项研究的目的是研究来自杏仁核，亚乳突/偏头（SC/PB）核的C1-C2区域过程信息中的前脊髓途径的神经元，以及模糊传入的纤维以调节脊髓胶中的感觉运动。我们先前证明，C1-C2神经元的化学刺激调节了腰椎脊柱神经元中自发性和内脏诱发的活性，以及​​胸部脊髓脊髓肌肉的EMG活性。我们的初步数据进一步表明，C1 C2神经元的化学刺激可以强烈影响与T3-T4呼吸系统中间神经元的活性。我们的初步结果尤其重要，这表明用依氏酸，减弱的杏仁核和SC/PB调节对C1-C2神经元的兴奋性毒性阻断。相同的病变减少了对腰椎神经元和脊髓脊髓肌肉的迷走神经作用。我们的结果挑战了以下假设：来自脊柱上部区域的下降途径仅通过直接投影调节胸神经和腰ac的神经元的活性。本应用程序解决了以下假设：C1-C2神经元从Amygdala，SC/PB和迷走神经传入中处理信息。反过来，C1-C2神经元强烈影响脊柱感觉神经元，胸腔呼吸相关的中间神经元和体形反射的活性。我们还假设杏仁核和迷走神经传入将信息通过SC/PB核传输到C1-C2神经元。具体目的旨在回答以下问题：1）是否受刺激特定的脊柱上核影响的C1-C2神经元的放电模式和活性？ 2）C1-C2片段中的神经元是否会从特定的脊柱上核处理信息，以改变脊髓中的感觉和积分/运动活性？ 3）C1-C2段中的神经元是否会从迷走神经传入中处理信息，以改变脊髓中的整合/运动和感觉活动？ 4）从杏仁核和迷走神经传入纤维到C1-C2段的SC/PB核中继信息是否会改变脊髓中的感觉和积分/运动活动？神经生理研究将在麻醉大鼠中检查细胞外放电模式和使用C-FOS作为神经元激活标志的研究。