Regulating the balance between resistance and infection

调节抵抗力与感染之间的平衡

• 批准号: 6500540
• 负责人: CHRISTINE A. BIRON
• 金额: $ 23.66万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6500540
• 关键词: B lymphocyte; Betaherpesvirinae; MHC class I antigen; Polyomavirus hominis 2; Toxoplasma gondii; cellular immunity; hepatitis B virus group; laboratory mouse; lymphocytic choriomeningitis virus; microorganism immunology; molecular genetics; natural killer cells; ribonuclease H; tissue /cell culture; virus protein

The studies proposed in PROJECT A seek to develop scientific tools to characterize molecular mechanisms regulating balance between defense mediated by the immune system and establishment or maintenance of infections. The PI is the chairperson of the Department of Molecular Microbiology and Immunology (MMI), Christine A. Biron. The experiments proposed are from Assistant Professors in the Department, Walter J. Altwood, Andrew G. Campbell, Laurent Brossay, and George Yap. MMI is the youngest of the basic science departments at Brown University. It is developing a focus on the programmatic theme presented in this application, and the overall general research area of innate mechanisms in resistance to microbial infections. The MMI group's interest compliment research in a number of clinical departments. All of the junior investigators in this project will benefit from the efforts proposed for faculty and research development in the administrative core. The studies planned will take advantage of and dependent upon the research core facilities proposed including the transgenic mouse, flow cytometric, and confocal/imaging laboratories. The work will be advanced in the following specific aims: 1. Establishment of Approaches to Identify Early Events in Infection of B cells by JV Virus, an Agent Associated with Neuropathology, 2. Characterization of the Structure and Function of the Hepatitis B Virus RNase H Enzyme Using a Novel Molecular Approach, 3. Development of a System to Critically Test the Function of NK T Cells in Responses to Infectious Agents, and 4. Construction of a System to Characterize Tissue-Specific Regulation of MHC Class I Expression as a Determinant of Latency and Immunopathology in Toxoplasma. The products and results of these experiments promise to advance understanding of basic molecular events contributing to health and disease during infections. Moreover, they will enhance Rhode Island's competitiveness for further national research support by developing these junior investigators and by providing them with unique new experimental tools for use in other advancing and developing projects characterizing the factors regulating and therapeutic tools for enhancing defense against infections.

项目 A 中提出的研究旨在开发科学工具来表征调节免疫系统介导的防御与感染的建立或维持之间平衡的分子机制。 PI 是分子微生物学和免疫学 (MMI) 系的系主任 Christine A. Biron。提议的实验由该系助理教授 Walter J. Altwood、Andrew G. Campbell、Laurent Brossay 和 George Yap 提出。 MMI 是布朗大学最年轻的基础科学系。它正在重点关注本申请中提出的计划主题，以及抵抗微生物感染的先天机制的总体研究领域。 MMI 小组对许多临床科室的研究感兴趣。该项目的所有初级研究人员都将受益于行政核心的师资和研究发展的努力。计划的研究将利用并依赖于拟议的研究核心设施，包括转基因小鼠、流式细胞术和共聚焦/成像实验室。这项工作将朝着以下具体目标推进：1. 建立识别 B 细胞感染 JV 病毒（一种与神经病理学相关的病原体）早期事件的方法，2. 乙型肝炎病毒 RNase H 的结构和功能表征使用新型分子方法的酶，3. 开发一个系统来严格测试 NK T 细胞对感染因子反应的功能，以及 4. 构建表征 MHC I 类表达的组织特异性调节作为弓形虫潜伏期和免疫病理学决定因素的系统的研究。这些实验的产品和结果有望增进对感染期间影响健康和疾病的基本分子事件的理解。此外，他们将通过培养这些初级研究人员并为他们提供独特的新实验工具以用于其他推进和开发项目，以增强罗德岛州在进一步国家研究支持方面的竞争力，以增强对感染的防御能力。