DRUG RESISTANCE IN MALARIA

疟疾的耐药性

• 批准号: 6583178
• 负责人: Carol Hopkins Sibley
• 金额: $ 1.66万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-15 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6583178
• 关键词: Plasmodium falciparum; Saccharomyces cerevisiae; antimalarial agents; combination chemotherapy; dihydrofolate reductase; drug hypersensitivity; drug interactions; drug resistance; drug screening /evaluation; enzyme inhibitors; enzyme structure; field study; gene expression; human tissue; malaria; point mutation; protein structure function; sulfur compounds; thymidylate synthase

DESCRIPTION (Adapted from the Applicant's Abstract): Dr. Sibley and coworkers have expressed both dihydrofolate reductase and dihydropteroate synthase from Plasmodium falciparum in the budding yeast, Saccharomyces cerevisiae and shown that one can study the parasite enzymes in this simple system. When completed their experiments will provide a detailed understanding of the mutations that modify function of the Pf-DHFR and Pf-DHPS enzymes or sensitivity of the enzymes to antifolate drugs.

描述（改编自申请人的摘要）：Sibley 博士和 同事表达了二氢叶酸还原酶和二氢叶酸 来自芽殖酵母、酵母菌中的恶性疟原虫的合酶 酿酒酵母并表明人们可以在这种简单的方法中研究寄生虫酶 系统。 完成后，他们的实验将提供详细的 了解改变 Pf-DHFR 功能的突变和 Pf-DHPS 酶或酶对抗叶酸药物的敏感性。

项目成果

Rare, highly pyrimethamine-resistant alleles of the Plasmodium falciparum dihydrofolate reductase gene from 5 African sites.

来自 5 个非洲地点的恶性疟原虫二氢叶酸还原酶基因的罕见、高度乙胺嘧啶抗性等位基因。

• DOI: 10.1086/425078
• 发表时间: 2004
• 期刊: The Journal of infectious diseases.
• 作者: Bates,SarahJ;Winstanley,PeterA;Watkins,WilliamM;Alloueche,Ali;Bwika,Juma;Happi,TChristian;Kremsner,PeterG;Kublin,JamesG;Premji,Zul;Sibley,CarolHopkins
• 通讯作者: Sibley,CarolHopkins

Plasmodium falciparum: in vitro activity of sulfadoxine and dapsone in field isolates from Kenya: point mutations in dihydropteroate synthase may not be the only determinants in sulfa resistance.

恶性疟原虫：来自肯尼亚的现场分离株中磺胺多辛和氨苯砜的体外活性：二氢蝶酸合酶的点突变可能不是磺胺耐药性的唯一决定因素。

• DOI: 10.1016/s0014-4894(02)00108-x
• 发表时间: 2002
• 期刊: Experimental parasitology
• 影响因子: 2.1
• 作者: Mberu,EdwardK;Nzila,AlexisM;Nduati,Eunice;Ross,Amanda;Monks,StephanieM;Kokwaro,GilbertO;Watkins,WilliamM;HopkinsSibley,Carol
• 通讯作者: HopkinsSibley,Carol