In Vitro Differentiation of Inner Ear Hair Cells

内耳毛细胞的体外分化

• 批准号: 6446493
• 负责人: Patricia M. White
• 金额: $ 3.48万
• 依托单位: HOUSE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6446493
• 关键词: biological signal transduction; bromodeoxyuridine; cell cycle; cell differentiation; deafness; ear hair cell; flow cytometry; genetically modified animals; green fluorescent proteins; immunofluorescence technique; laboratory mouse; labyrinth; nervous system regeneration; tissue /cell culture

DESCRIPTION (provided by applicant): Sensorineural deafness in adults is primarily due to the permanent loss of sensory hair cells in the inner ear. In this regard, mammals differ from lower vertebrates, which can regenerate sensory hair cells from adjacent supporting cells after injury. It is not known whether this difference arises from a lack of sensory developmental capacity in mammalian support cells, or from a lack of some endogenous stimulus in mammals to provoke regeneration after otic injury. Experiments described in this proposal will mark embryonic sensory and support cell precursors with a fluorescent transgene. These precursors will be purified from embryos using fluorescence activated cell sorting (FACS) and differentiated in vitro, using a recently developed culture system. This system will also be used to isolate and differentiate post-mitotic support cells from embryos and from post-natal animals. From these experiments, it will be determined whether post-mitotic mammalian support cells are able to generate sensory hair cells, and if so, how long in development this capacity persists.

描述（由申请人提供）： 成人感音神经性耳聋是 主要是由于内耳感觉毛细胞的永久丧失。在 在这方面，哺乳动物与低等脊椎动物不同，低等脊椎动物可以再生感觉 受伤后来自邻近支持细胞的毛细胞。尚不清楚是否 这种差异是由于缺乏感觉发育能力造成的 哺乳动物支持细胞，或由于哺乳动物缺乏某些内源性刺激 促进耳损伤后的再生。本文描述的实验 该提案将标记胚胎感觉并支持细胞前体 荧光转基因。这些前体将使用以下方法从胚胎中纯化： 荧光激活细胞分选（FACS）并在体外进行分化，使用 最近开发的文化系统。该系统还将用于隔离和 区分胚胎和出生后的有丝分裂后支持细胞 动物。从这些实验中，将确定是否有丝分裂后 哺乳动物支持细胞能够产生感觉毛细胞，如果是的话，是如何产生的 在长期的发展过程中，这种能力仍然存在。