ECDYSONE RECEPTOR REQUIREMENTS IN DROSOPHILA DEVELOPMENT

果蝇发育中的蜕皮激素受体需求

• 批准号: 6329595
• 负责人: Melissa B Davis
• 金额: $ 2.19万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-12-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6329595
• 关键词: Drosophilidae; biological signal transduction; ecdysone; gene expression; gene interaction; gene mutation; growth /development; hormone binding protein; hormone receptor; hormone regulation /control mechanism; immunocytochemistry; in situ hybridization; northern blottings; nucleic acid sequence; protein isoforms; protein structure function; western blottings; Drosophilidae; biological signal transduction; ecdysone; gene expression; gene interaction; gene mutation; growth /development; hormone binding protein; hormone receptor; hormone regulation /control mechanism; immunocytochemistry; in situ hybridization; northern blottings; nucleic acid sequence; protein isoforms; protein structure function; western blottings

Hormone signaling is required in a variety of developmental processes in a host of animals, from insects to humans. Defects in hormones and their receptors are associated with many medical defects and diseases, including cancers, developmental and reproductive disorders. The receptors of these hormones function as ligand activated transcriptional regulators (Evans, 1988). The fact that these hormone molecules initiate so many specialized and tightly regulated mechanisms, makes the investigation and elucidation of these pathways an extremely complicated endeavor. For this task the Drosophila animal system has been recruited for its economical and efficient means of genetic manipulation. The steroid hormone ecdysone acts to trigger molting and metamorphosis during Drosophila development. Ecdysone pulses occur at very specific intervals and at definite concentration levels during Drosophila development. The only known receptor molecule for ecdysone, ecdysone receptor protein (EcR), is a nuclear receptor that [along with its heterodimeric partner, ultraspiracle protein (USP)] binds ecdysone and promotes the transcription of target genes. There are at least three isoforms of the EcR protein, (EcR-A, EcR-B1, and EcR-B2) and each is hypothesized to have separate spatial and/or temporal activities, based on their unique expression patterns (Talbot. 1993), and their unique mutant phenotype analyses (Bender, 1997). This data suggests that the isoforms play a key role in the coordination of the correct signaling paths induced by ecdysone. The goals of this project are to molecularly determine the requirements of the EcR-A during Drosophila melanogaster development.

从昆虫到人类的各种动物中，各种发育过程中都需要激素信号传导。 激素及其受体的缺陷与许多医学缺陷和疾病有关，包括癌症，发育和生殖疾病。 这些激素的受体充当配体激活的转录调节剂（Evans，1988）。这些激素分子启动了许多专业和严格调节的机制，这一事实使对这些途径的研究和阐明是极其复杂的努力。 对于这项任务，果蝇动物系统的经济和有效的遗传操作手段已被招募。 果蝇发育过程中，类固醇激素condyson可引发摩擦和变形。在果蝇发育过程中，ecdysone脉冲以非常具体的间隔和明确的浓度水平出现。 Ecdysone，Ecdysone受体蛋白（ECR）的唯一已知的受体分子是一种核受体，它[与其异二二聚体伴侣Ultraspiracle蛋白（USP）一起结合ecdysone并促进靶基因的转录。 ECR蛋白（ECR-A，ECR-B1和ECR-B2）至少有三种同工型，并且根据其独特的表达模式（Talbot。1993）及其独特的突变表型分析（Bender，1997），每个ECR蛋白（ECR-A，ECR-B1和ECR-B2）至少具有单独的空间和/或时间活动。 该数据表明，同工型在ecdysone引起的正确信号通路的配位中起关键作用。 该项目的目标是分子确定果蝇果蝇开发过程中ECR-A的需求。