CORE--MICROARRAY

核心--微阵列

• 批准号: 6501421
• 负责人: William Wachsman
• 金额: $ 18.37万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-21 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6501421
• 关键词: biomedical facility; microarray technology

The worldwide genomics effort is poised to yield insights into numerous diseases, including cancer. New technologies are being implemented to more effectively exploit these massive amounts of genetic data that will undoubtedly increase several fold in the near future. To this end, microarrays have emerged as a powerful tool for simultaneous examination of thousands of genetic measurements. It is anticipated that microarray technology will play a significant role in cancer research to characterize underlying mechanisms, develop molecular diagnostics/prognostics, and eventually facilitate cancer therapeutic decision-making. The Microarray Shared Resource was developed to provide Center investigators with this technology that heretofore has been available on a very limited basis in academia. Current resources include an Affymetrix GeneChip system with which assays for RNA expression; resequencing of p53, HIV protease/reverse transcriptase, and cytochrome p450; or determination of single nucleotide polymorphisms can be performed using specialized high density oligonucleotide-based GeneChip arrays. To complement the GeneChip approach for RNA expression assays, the Resource has also partnered with the UCSD BIOGEM Lab to produce and assay spotted glass-slide microarrays. In addition to performing microarray assays, the Resource provides expertise in experimental design microarrays. In addition, an Array Science Journal Club and Workshops has been implemented to educate and train investigators in this rapidly evolving field. This Resource is instrumental to investigators conducting either or both basic and clinical cancer research in all of the Center Programs. It also enables the development of intra- and inter-Programmatic interactions throughout the Center and encourage others, not currently performing cancer research to become involved in Center activities.

全球基因组学的努力有望对包括癌症在内的众多疾病产生见解。正在实施新技术，以更有效地利用这些大量的遗传数据，这些数据无疑会在不久的将来增加几倍。为此，微阵列已成为同时研究数千种遗传测量的有力工具。预计微阵列技术将在癌症研究中发挥重要作用，以表征潜在的机制，发展分子诊断/预后，并最终促进癌症治疗决策。微阵列共享资源的开发是为了为中心调查人员提供迄今为止在学术界非常有限的技术。当前的资源包括一个Affymetrix Genechip系统，用于RNA表达的测定； p53，HIV蛋白酶/逆转录酶和细胞色素P450的重新陈述；或可以使用专门的高密度寡核苷酸的转基因阵列来确定单核苷酸多态性。为了补充RNA表达测定的Genechip方法，该资源还与UCSD BioGem Lab合作，生产和测定斑点的玻璃滑雪微阵列。除了执行微阵列测定外，该资源还提供了实验设计微阵列的专业知识。此外，已经实施了阵列科学杂志俱乐部和研讨会，以在这个迅速发展的领域进行教育和培训调查人员。该资源对所有中心计划中进行基础癌症和临床癌症研究的研究人员起着重要作用。它还可以在整个中心开发整个中心的跨编程间相互作用，并鼓励其他人，目前没有进行癌症研究以参与中心活动。