REGULATION OF ASYMMETRIC FLAGELLAR WAVEFORMS

不对称鞭毛波形的调节

• 批准号: 6386065
• 负责人: PAUL A. LEFEBVRE
• 金额: $ 21.38万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6386065
• 关键词: Chlamydomonas; cilium /flagellum motility; gene mutation; immunoprecipitation; microtubule associated protein; molecular cloning; protein binding; protein sequence; protein structure function; site directed mutagenesis; yeast two hybrid system

The investigator's goal is to understand the mechanism and regulation of flagellar motility. They have taken advantage of the powerful genetic tools available in Chlamydomonas, coupled with newly developed techniques to tag and clone genes by insertional mutagenesis, to begin a molecular dissection of function of the central pair complex of the flagellar axoneme. The specific aims are: 1) to clone and characterize additional genes for central apparatus components; 2) to identify proteins interacting with components of the central apparatus; 3) to define functional domains required for assembly and function of individual central apparatus components using in vitro mutagenesis. The amramdillo repeat protein PF16, will be the first target for detailed molecular analysis. The investigator states that the study of central apparatus components will not only have an impact on the field of flagellar motility but also on the fields of motor regulation and microtubule function.

研究者的目标是了解机制和法规 鞭毛运动。 他们利用了强大的 衣原体可用的遗传工具，再加上新开发的 通过插入诱变来标记和克隆基因的技术，开始 中央对络合物功能的分子解剖 鞭毛轴突。具体目的是：1）克隆和表征 中央设备成分的其他基因； 2）识别 蛋白质与中央设备的成分相互作用； 3）到 定义组装和功能所需的功能域 使用体外诱变的单个中央设备组件。 这 Amramdillo重复蛋白PF16将是详细的第一个目标 分子分析。 研究者指出，中央的研究 设备组件不仅会对现场产生影响 鞭毛运动性，但在电动机调节和 微管功能。

项目成果

Regulation of flagellar dynein by calcium and a role for an axonemal calmodulin and calmodulin-dependent kinase.

钙对鞭毛动力蛋白的调节以及轴丝钙调蛋白和钙调蛋白依赖性激酶的作用。

• DOI: 10.1091/mbc.e02-04-0185
• 发表时间: 2002
• 期刊: Molecular biology of the cell
• 影响因子: 3.3
• 作者: Smith,ElizabethF

PF16 encodes a protein with armadillo repeats and localizes to a single microtubule of the central apparatus in Chlamydomonas flagella.

• DOI: 10.1083/jcb.132.3.359
• 发表时间: 1996-02
• 期刊: The Journal of cell biology
• 作者: Smith EF;Lefebvre PA
• 通讯作者: Lefebvre PA