EFFECTS OF ALCOHOL ON MOLECULAR AND CELLULAR PATHOLOGY

酒精对分子和细胞病理学的影响

• 批准号: 6509150
• 负责人: ESTEBAN MEZEY
• 金额: $ 7.94万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-30 至 2004-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6509150
• 关键词: EFFECTS; ALCOHOL; MOLECULAR; CELLULAR; PATHOLOGY

The research training objective is to provide a proper environment for the development of independent investigators in the field of alcoholism as it relates to molecular and cellular pathology. Post-doctoral fellows will work closely with a preceptor on a project related to the research of the preceptor. The trainees will interact among themselves, with other preceptors in the program, and with the research community as a whole of the Institution. Also, they can obtain formal instruction in biochemistry, biostatistics, and research methodology, and will be exposed to lectures and interaction with established investigators in the field of alcoholism. The principal areas and significance of the research training offered are: 1. Hormonal regulation of the rat class I alcohol dehydrogenase (ADH). This study identifies the cis- and trans- acting elements that mediate the effect of hormones on the ADH gene. 2. Mechanisms of liver fibrogenesis. Investigation of factors that activate stellate cells to collagen producing myofibroblast-like cell and mechanisms whereby acetaldehyde stimulates fibrogenesis. 3. Alterations in water transport across membranes. Study of effects of ethanol on aquaporin water transport proteins and water permeability across cells. 4. Effects of ethanol on hepatic regenerative growth. Studies of possible factors that may accelerate recovery from ethanol-induced liver injury. 5. Differences in dynamics of the hypothalamic-pituitary-adrenal axis as a risk factor for alcoholism. 6. Molecular and biological analysis of adenyl cyclase in alcoholism. These studies include a search for markers of predisposition towards alcoholism. 7. Regulation of growth and differentiation of intestinal epithelial cells. Study of effects of ethanol in intestinal cell differentiation and function. These areas of research will provide extensive training in different methods of biochemistry, molecular biology and cell culture. Trainees will be exposed to the application of basic research to studies of patients with alcoholism. The final objective is to instill in the trainee the ability to approach original questions of research relating to the pathogenesis of damaging effects of alcoholism on the body.

研究培训目标是为与分子和细胞病理有关的酗酒领域的独立研究人员提供适当的环境。博士后研究员将与一个与主持人研究有关的项目密切合作。学员将与计划中的其他受体以及整个机构的研究社区进行互动。此外，他们可以获得生物化学，生物统计学和研究方法的正式教学，并将暴露于讲座并与酒精中毒领域已建立的研究人员的互动。提供的研究培训的主要领域和意义是：1。大鼠I类酒精脱氢酶（ADH）的激素调节。这项研究确定了介导激素对ADH基因作用的顺式和转移元件。 2。肝纤维发生的机制。研究激活星状细胞以产生肌纤维细胞样细胞的因素和机制，从而使乙醛刺激纤维发生。 3。跨膜的水运输改变。研究乙醇对水库水的影响跨细胞的蛋白质和渗透性的研究。 4。乙醇对肝再生生长的影响。研究可能加速乙醇诱导肝损伤的可能因素的研究。 5。下丘脑 - 垂体 - 肾上腺轴的动力学差异是酒精中毒的危险因素。 6。酒精中毒中腺基环化酶的分子和生物学分析。这些研究包括寻找对酒精中毒倾向的标记。 7。调节肠上皮细胞的生长和分化。研究乙醇在肠细胞分化和功能中的影响。这些研究领域将提供有关生物化学，分子生物学和细胞培养方法的不同方法的广泛培训。受训者将暴露于基础研究中，将其应用于酒精中毒患者的研究。最终的目标是向受训者灌输与酒精中毒对身体损害影响的发病机理有关的原始研究问题的能力。